School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Photodiagnosis Photodyn Ther. 2024 Oct;49:104241. doi: 10.1016/j.pdpdt.2024.104241. Epub 2024 Aug 20.
Photodynamic therapy (PDT), as one of the most promising cancer therapy methods, is still limited by several drawbacks, such as tissue hypoxia and shallow light penetration of blue-violet light (200-450 nm), and red light (750 nm) is more penetrating to tissues than blue-violet light, but still lower than near-red light (750-1350 nm). Therefore, we proposed a synergistic therapy system by combining the near-infrared light-triggered PDT with nitric oxide (NO)-based gas therapy to enhance the anti-tumor effects. Upconversion nanoparticles (UCNPs) were loaded with the photosensitizers of ZnPc and the NO donors of l-arginine (L-Arg) to obtain the nanocomposites of UCN@mSiO@ZnPc@L-Arg. Under 980 nm laser irradiation, reactive oxygen species (ROS) could be produced for PDT and react with l-Arg to produce NO, which is previously reported to have a greater killing effect on tumor cells than ROS and also plays an important role in promoting PDT in our study. Both the in vitro and in vivo tests demonstrated that the combined therapy of PDT with NO therapy could enhance the tumor killing effect significantly compared with the unique application of PDT. The UCNPs-based nanocomposites are expected to be widely used in biomedicine for tumor inhibition.
光动力疗法（PDT）作为最有前途的癌症治疗方法之一，仍然受到一些缺点的限制，如组织缺氧和蓝紫光（200-450nm）的浅层光穿透性，而红光（750nm）比蓝紫光更能穿透组织，但仍低于近红光（750-1350nm）。因此，我们提出了一种协同治疗系统，将近红外光触发的 PDT 与基于一氧化氮（NO）的气体治疗相结合，以增强抗肿瘤效果。上转换纳米粒子（UCNP）负载光敏剂 ZnPc 和 NO 供体 l-精氨酸（L-Arg），得到 UCN@mSiO@ZnPc@L-Arg 纳米复合材料。在 980nm 激光照射下，可产生用于 PDT 的活性氧（ROS），并与 L-Arg 反应生成 NO，据先前报道，NO 对肿瘤细胞的杀伤作用比 ROS 更大，在我们的研究中也对促进 PDT 发挥着重要作用。体外和体内实验均表明，与单独应用 PDT 相比，NO 治疗联合 PDT 的联合治疗能显著增强肿瘤杀伤效果。基于 UCNP 的纳米复合材料有望在肿瘤抑制的生物医学领域得到广泛应用。
