Biomaterials Group, Materials Design Division, Faculty of Materials Science and Engineering, Warsaw University of Technology, Woloska 141, 02-507 Warsaw, Poland.
Institute of Metallic Biomaterials, Helmholtz-Zentrum Hereon GmbH, 21502 Geesthacht, Germany.
Acta Biomater. 2024 Oct 1;187:471-490. doi: 10.1016/j.actbio.2024.08.015. Epub 2024 Aug 19.
In this study, the interaction of pure Mg and WE43 alloy under the presence of osteoblast (OB) and osteoclast (OC) cells and their influence on the degradation of materials have been deeply analyzed. Since OB and OC interaction has an important role in bone remodeling, we examined the surface morphology and dynamic changes in the chemical composition and thickness of the corrosion layers formed on pure Mg and WE43 alloy by direct monoculture and coculture of pre-differentiated OB and OC cells in vitro. Electrochemical techniques examined the corrosion performance. The corrosion products were characterized using a combination of the focused ion beam (FIB), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). Cell viability and morphology were assessed by fluorescent microscopy and SEM. Our findings demonstrate cell spread and attachment variations, which differ depending on the Mg substrates. It was clearly shown that cell culture groups delayed degradation processes with the lowest corrosion rate observed in the presence of OBOC coculture for the WE43 substrate. Ca-P enrichment was observed in the outer-middle region of the corrosion layer but only after 7 days of OBOC coculture on WE43 and after 14 days on the pure Mg specimens. STATEMENT OF SIGNIFICANCE: Magnesium metallic materials that can degrade over time provide distinct opportunities for orthopedic application. However, there is still a lack, especially in elucidating cell-material interface characterization. This study investigated the influence of osteoblast-osteoclast coculture in direct Mg-material contact. Our findings demonstrated that pre-differentiated osteoblasts and osteoclasts cocultured on Mg substrates influenced the chemistry of the corrosion layers. The cell spread and attachment were Mg substrate-dependent. The findings of coculturing bone cells directly on Mg materials within an in vitro model provide an effective approach for studying the dynamic degradation processes of Mg alloys while also elucidating cell behavior and their potential contribution to the degradation of these alloys.
在这项研究中，深入分析了在成骨细胞（OB）和破骨细胞（OC）存在的情况下纯镁和 WE43 合金的相互作用及其对材料降解的影响。由于 OB 和 OC 的相互作用在骨重塑中起着重要作用，我们通过体外直接单核培养和共培养预分化的 OB 和 OC 细胞，检查了纯镁和 WE43 合金表面形貌和化学成分及腐蚀层厚度的动态变化。电化学技术检测了腐蚀性能。采用聚焦离子束（FIB）、扫描电子显微镜（SEM）和能谱（EDX）相结合的方法对腐蚀产物进行了表征。通过荧光显微镜和 SEM 评估了细胞活力和形态。我们的研究结果表明，细胞的扩散和附着存在差异，这取决于镁基底。很明显，细胞培养组延迟了降解过程，在 WE43 基底上 OBOC 共培养时观察到最低的腐蚀速率。在 WE43 上共培养 7 天后和纯镁标本上共培养 14 天后，在腐蚀层的中外层区域观察到 Ca-P 富集。 意义声明：能够随时间降解的镁金属材料为骨科应用提供了独特的机会。然而，特别是在阐明细胞-材料界面特性方面，仍然存在不足。本研究探讨了成骨细胞-破骨细胞共培养对直接 Mg 材料接触的影响。我们的研究结果表明，预分化的成骨细胞和破骨细胞共培养在 Mg 基底上影响了腐蚀层的化学性质。细胞的扩散和附着依赖于 Mg 基底。在体外模型中直接将骨细胞共培养在 Mg 材料上的研究结果为研究镁合金的动态降解过程提供了有效的方法，同时阐明了细胞行为及其对这些合金降解的潜在贡献。
