Yang Tang, Wang Xu-Yi, Sui Xin, Hui Xiao-Liang, Wang Zhen-Xing, Jiang Bo, Zhang Zhan-Peng, Li Xin-Long
School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, China.
Development Construction Co., Ltd., China Construction Eighth Engineering Division, Qingdao 266061, China.
Huan Jing Ke Xue. 2024 Aug 8;45(8):4512-4519. doi: 10.13227/j.hjkx.202309002.
To explore the prevalence and source of antibiotic resistant genes (ARGs) and pathogenic antibiotic resistant bacteria (PARB) associated with bioaerosols in wastewater treatment plants (WWTPs), metagenomic sequencing and assembly were applied to elucidate the antibiotic resistome of bioaerosols and wastewater in WWTPs. The results showed that more subtypes of ARGs and a higher abundance of PARB were found in bioaerosols from WWTPs and downwind than those from upwind. Multidrug and were respectively the most dominant type and subtype of ARGs in bioaerosols from WWTPs. In total, 37 types of PARB carried at least two or more ARG types and were characterized by multiple drug resistance. At the fine grid, aerated tank, and sludge dewatering room, wastewater was the main source of bioaerosol ARGs and PARB. A total of 32 PARB were easily aerosolized in at least one wastewater treatment unit, such as and . This study will provide theoretical support for the risk assessment and health protection of antibiotic resistant pollution associated with bioaerosols from WWTPs.
为了探究污水处理厂(WWTPs)中与生物气溶胶相关的抗生素抗性基因(ARGs)和致病性抗生素抗性细菌(PARB)的流行情况及来源,采用宏基因组测序和组装技术来阐明污水处理厂中生物气溶胶和废水的抗生素抗性组。结果表明,污水处理厂及下风向生物气溶胶中发现的ARGs亚型更多,PARB丰度也高于上风向。多药抗性分别是污水处理厂生物气溶胶中最主要的ARGs类型和亚型。共有37种PARB携带至少两种或更多种ARG类型,并具有多重耐药性。在细格栅、曝气池和污泥脱水间,废水是生物气溶胶ARGs和PARB的主要来源。共有32种PARB在至少一个废水处理单元(如……)中容易被气溶胶化。本研究将为污水处理厂生物气溶胶相关抗生素抗性污染的风险评估和健康防护提供理论支持。