Eurotransplant Reference Laboratory, Leiden University Medical Center, Leiden, the Netherlands.
DKMS Group, Tübingen, Germany.
HLA. 2024 Aug;104(2):e15653. doi: 10.1111/tan.15653.
On 24 January 2023, Eurotransplant has introduced the virtual crossmatch for kidney and pancreas allocation as a better alternative for the physical Complement Dependent Cytotoxicity (CDC) crossmatches at the donor centre, which were associated with a longer cold ischaemia time and false positive reactions. For the time being, the physical CDC crossmatch at the recipient centre will remain in place as the final histocompatibility check. While Eurotransplant is certainly not the first organ allocation organisation to introduce virtual crossmatching, several novel aspects have been introduced, such as calculation of the virtual panel reactive antibody (vPRA) on 11 loci at the second-field level in addition to the serological broad and split level, electronic HLA typing data transmission using Histoimmunogenetics Markup Language (HML) file format, and the actual virtual crossmatch based on ambiguous, second-field HLA typing of the donor on all 11 loci. This short communication will focus on these novel aspects of the virtual crossmatch in Eurotransplant.
2023 年 1 月 24 日，欧洲器官移植组织（Eurotransplant）推出了虚拟交叉配型，以替代在供体中心进行的实体补体依赖性细胞毒性（CDC）交叉配型，因为实体 CDC 交叉配型会导致更长的冷缺血时间和假阳性反应。目前，受体中心的实体 CDC 交叉配型仍将作为最终的组织相容性检查。虽然 Eurotransplant 肯定不是第一个引入虚拟交叉配型的器官分配组织，但它引入了一些新的方面，例如在第二领域水平上计算 11 个位点的虚拟 panel reactive antibody (vPRA)，除了血清学广泛和分裂水平外，还使用组织免疫遗传学标记语言 (HML) 文件格式传输电子 HLA 分型数据，以及基于供体所有 11 个位点的模糊、第二领域 HLA 分型的实际虚拟交叉配型。本简讯将重点介绍 Eurotransplant 中虚拟交叉配型的这些新方面。
