用于潜在生物成像和治疗的脂质包覆碳量子点特性中的细胞摄取和活力转换

Cellular uptake and viability switching in the properties of lipid coated carbon quantum dots for potential bioimaging and therapeutics.

作者信息

Jain Sweny, Sahu Nidhi, Bhatia Dhiraj, Yadav Pankaj

机构信息

Department of Biological Sciences and Engineering, Indian Institute of Technology Gandhinagar Palaj Gujarat 382355 India

Department of Bioengineering and Biotechnology, Birla Institute of Technology Mesra Ranchi Jharkhand-835 215 India.

出版信息

Nanoscale Adv. 2024 Aug 7;6(20):5069-79. doi: 10.1039/d4na00306c.

DOI:10.1039/d4na00306c

PMID:39170765

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11333955/

Abstract

Carbon quantum dots derived from mango leaves exhibited red fluorescence. These negatively charged particles underwent coating with the positively charged lipid molecule -[1-(2,3-dioleyloxy)propyl]-,,-trimethylammonium chloride (DOTMA). However, the bioconjugate displayed reduced uptake compared to the standalone mQDs in cancer cells (SUM 159A), and increased uptake in case of non-cancerous (RPE-1) cells. Upon testing, the bioconjugate demonstrated a mitigating effect on the individual toxicity of both DOTMA and mQDs in SUM-159 (cancerous cells). Conversely, it exhibited a proliferative effect on RPE-1 (normal cells).

摘要

从芒果叶中提取的碳量子点发出红色荧光。这些带负电荷的粒子用带正电荷的脂质分子——[1-(2,3-二油酰氧基)丙基]三甲基氯化铵（DOTMA）进行了包覆。然而，与单独的芒果叶碳量子点（mQDs）相比，这种生物共轭物在癌细胞（SUM 159A）中的摄取减少，而在非癌细胞（RPE-1）中摄取增加。经测试，这种生物共轭物对SUM-159（癌细胞）中DOTMA和mQDs的个体毒性具有减轻作用。相反，它对RPE-1（正常细胞）表现出增殖作用。