Moore H D, Hartman T D, Brown A C, Smith C A, Ellis D H
MRC/AFRC Comparative Physiology Group, Institute of Zoology, Zoological Society of London, UK.
Exp Clin Immunogenet. 1985;2(2):84-96.
The expression of hamster sperm antigens was investigated during spermatogenesis and sperm maturation with the use of monoclonal antibodies generated in culture from mice immunized with hamster cauda epididymal spermatozoa or sperm heads. Antigens were localized by immunofluorescence and immunoperoxidase techniques which were first visualized on isolated spermatids over the developing acrosome. In one case, antibody inhibited fertilization in vitro although localization on testicular or epididymal spermatozoa was minimal compared with the early spermatid. Antibodies also recognized surface antigens first expressed in the epididymis whose localization on the spermatozoon altered during epididymal transit or incubation in capacitating medium. The results were discussed in relation to the expression and function of surface determinants on the haploid germ cell.
利用用仓鼠附睾尾精子或精子头部免疫的小鼠培养产生的单克隆抗体，研究了仓鼠精子抗原在精子发生和精子成熟过程中的表达。通过免疫荧光和免疫过氧化物酶技术对抗原进行定位，这些技术首先在发育中的顶体上的分离精子细胞上可视化。在一个案例中，抗体抑制了体外受精，尽管与早期精子细胞相比，在睾丸或附睾精子上的定位最少。抗体还识别首先在附睾中表达的表面抗原，其在精子上的定位在附睾转运或在获能培养基中孵育期间发生改变。结合单倍体生殖细胞表面决定簇的表达和功能对结果进行了讨论。
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