评价姜黄和过氧化物酶体增殖物激活受体γ激动剂吡格列酮对百草枯诱导的大鼠肺损伤的保护作用。

BACKGROUND: The inhalation of paraquat (PQ), one of the most widely used herbicides in the world, can result in lung injury. Curcuma longa (Cl) has long history in traditional and folk medicine for the treatment of a wide range of disorders including respiratory diseases. AIM: The aim of the present work was to evaluate the preventive effect of Cl on inhaled PQ-induced lung injury in rats. METHODS: Male Wistar rats were divided into 8 groups (n = 7), one group exposed to saline (control) and other groups exposed to PQ aerosol. Saline (PQ), Cl extract, (two doses), curcumin (Cu), pioglitazone (Pio), and the combination of Cl-L + Pio and dexamethasone (Dex) were administered during the exposure period to PQ. Total and differential white blood cell (WBC) counts, oxidant and antioxidant indicators in the bronchoalveolar lavage (BALF), interleukin (IL)-10, and tumor necrosis alpha (TNF-α) levels in the lung tissues, lung histologic lesions score, and air way responsiveness to methacholine were evaluated. RESULTS: WBC counts (Total and differential), malondialdehyde level, tracheal responsiveness (TR), IL-10, TNF-α and histopathological changes of the lung were markedly elevated but total thiol content and the activities of catalase and superoxide dismutase were decreased in the BALF in the PQ group. Both doses of Cl, Cu, Pio, Cl-L + Pio, and Dex markedly improved all measured variables in comparison with the PQ group. CONCLUSION: CI, Pio, and Cl-L + Pio improved PQ-induced lung inflammation and oxidative damage comparable with the effects of Dex.

背景：百草枯（PQ）是世界上使用最广泛的除草剂之一，吸入后会导致肺损伤。姜黄（Cl）在传统和民间医学中用于治疗各种疾病已有很长的历史，包括呼吸系统疾病。

目的：本研究旨在评估 Cl 对吸入 PQ 诱导的大鼠肺损伤的预防作用。

方法：雄性 Wistar 大鼠分为 8 组（n=7），一组暴露于生理盐水（对照），其他组暴露于 PQ 气溶胶。在暴露于 PQ 期间，给予生理盐水（PQ）、Cl 提取物（两种剂量）、姜黄素（Cu）、吡格列酮（Pio）和 Cl-L+Pio 联合地塞米松（Dex）。评估支气管肺泡灌洗液（BALF）中的总白细胞和分类白细胞计数、氧化应激和抗氧化指标、肺组织中的白细胞介素（IL）-10 和肿瘤坏死因子-α（TNF-α）水平、肺组织的组织学病变评分以及气道对乙酰甲胆碱的反应性。

结果：BALF 中的白细胞计数（总计数和分类计数）、丙二醛水平、气道反应性（TR）、IL-10、TNF-α和肺组织的组织病理学变化显著升高，但 Cl、Cu、Pio、Cl-L+Pio 和 Dex 均能显著改善所有与 PQ 组相比，BALF 中的总硫醇含量和过氧化氢酶及超氧化物歧化酶的活性均降低。

结论：Cl、Pio 和 Cl-L+Pio 可改善 PQ 诱导的肺炎症和氧化损伤，其作用与 Dex 相当。