Department of Pharmaceutical Sciences, North South University, Dhaka 1229, Bangladesh.
ACS Appl Bio Mater. 2024 Sep 16;7(9):6313-6324. doi: 10.1021/acsabm.4c01129. Epub 2024 Aug 22.
Subcutaneous (SC) administration of chemotherapeutics combined with near-infrared (NIR) light activation can effectively target skin tumors by triggering localized drug release and enhancing cytotoxic effects. In this study, we developed NIR-responsive indocyanine green (ICG) and the chemotherapeutic agent doxorubicin (Dox) loaded into gelatin nanoparticles (NPs) for SC delivery in a skin tumor-bearing mouse model. Histological examination (hematoxylin and eosin staining) confirmed the successful delivery and swelling behavior of the Dox/ICG-loaded gelatin NPs at the SC site. In vitro and in vivo experiments demonstrated that NIR activation of the Dox/ICG-loaded gelatin NPs generated significant photothermal heat (48 and 46 °C), leading to targeted drug release and a substantial reduction in skin tumor size (from 15 to 3 mm). Our findings suggest that this dual-modality approach of SC chemotherapeutic administration and NIR-triggered photothermal therapy can concentrate cytotoxic drugs at the tumor site, offering a promising strategy for improving skin cancer treatment.
皮下（SC）给予化疗药物并结合近红外（NIR）光激活可以通过触发局部药物释放和增强细胞毒性作用来有效靶向皮肤肿瘤。在这项研究中，我们开发了近红外响应性吲哚菁绿（ICG）和化疗药物阿霉素（Dox）负载到明胶纳米颗粒（NPs）中，用于在皮肤肿瘤荷瘤小鼠模型中进行 SC 递药。组织学检查（苏木精和伊红染色）证实了 Dox/ICG 负载的明胶 NPs 在 SC 部位的成功递送和肿胀行为。体外和体内实验表明，Dox/ICG 负载的明胶 NPs 的 NIR 激活产生了显著的光热（48 和 46°C），导致靶向药物释放和皮肤肿瘤大小的显著减小（从 15 毫米减小到 3 毫米）。我们的研究结果表明，这种 SC 化疗药物给予和 NIR 触发的光热治疗的双重模式方法可以将细胞毒性药物集中在肿瘤部位，为改善皮肤癌治疗提供了一种有前途的策略。
