He Xia, Chen Weijun, Zhou Xiaoni, Hu Gang, Wei Jing, Liu Yan, Cai Liping, Zhang Zhi, Chen Tingtao
Department of Obstetrics & Gynecology, the First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, Jiangxi, China.
Department of Obstetrics & Gynecology, the Ninth Hospital in Nanchang and The Affiliated Hospital of Nanchang University, Nanchang, 330038, Jiangxi, China.
Probiotics Antimicrob Proteins. 2024 Aug 22. doi: 10.1007/s12602-024-10349-6.
Chronic endometritis (CE) is common in patients with infertility, and it is challenging to treat with antibiotics as bacteria often acquire resistance to the antibiotics, which leads to frequent recurrence of the condition. Probiotics, especially Lactobacillus species, are known for their usefulness in treating reproductive infections. This study evaluated Lactobacillus crispatus chen 01 (L. crispatus chen 01) isolated from healthy women who were 22-30 years old and married with children. In vitro experiments showed that L. crispatus chen 01 inhibited pathogens and reduced inflammation in CE mice by downregulating inflammatory proteins (TLR, MyD88, and p65/p-p65; L + Abx vs M, P < 0.01), improving histopathological features, and inhibiting bacterial growth. It also regulated endometrial processes, such as enhancing embryo implantation (BMP2 and Wnt4, L + Abx vs M, P < 0.01) via the Wnt/β-catenin pathway, leading to increased pregnancy rates (L + Abx vs M, 100% vs 0%) in mice. In clinical trials, L. crispatus chen 01 improved progesterone levels (P = 0.0038), pregnancy rates (C vs Abx + L. c, 76.19% vs 87.18%), and pathological changes in CE patients. The findings from this study identify the administration of L. crispatus chen 01 as a promising intervention for CE that could improve pregnancy rates.
慢性子宫内膜炎（CE）在不孕症患者中很常见，用抗生素治疗具有挑战性，因为细菌常常会对抗生素产生耐药性，这导致病情频繁复发。益生菌，尤其是乳酸杆菌属，以其在治疗生殖系统感染方面的作用而闻名。本研究评估了从22至30岁、已婚并有子女的健康女性中分离出的卷曲乳酸杆菌陈01（L. crispatus chen 01）。体外实验表明，卷曲乳酸杆菌陈01通过下调炎症蛋白（TLR、MyD88和p65/p-p65；L+Abx组与M组相比，P<0.01）、改善组织病理学特征和抑制细菌生长，从而抑制病原体并减轻CE小鼠的炎症。它还通过Wnt/β-连环蛋白途径调节子宫内膜过程，如增强胚胎着床（BMP2和Wnt4，L+Abx组与M组相比，P<0.01），导致小鼠妊娠率增加（L+Abx组与M组相比，分别为100%和0%）。在临床试验中，卷曲乳酸杆菌陈01改善了CE患者的孕酮水平（P=0.0038）、妊娠率（C组与Abx+L. c组相比，分别为76.19%和87.18%）以及病理变化。本研究结果表明，给予卷曲乳酸杆菌陈01是一种有前景的CE干预措施，可提高妊娠率。
