Gross K M, Matsumoto A M, Southworth M B, Bremner W J
J Clin Endocrinol Metab. 1985 Jan;60(1):197-202. doi: 10.1210/jcem-60-1-197.
To examine the hypothesis that the frequency of endogenous pulsatile LHRH stimulation controls the relative secretion of FSH and LH from the pituitary, we studied men with elevated FSH levels and normal LH levels to determine whether they have an altered frequency of pulsatile LHRH secretion compared to normal men. Because peripheral blood measurements of LHRH do not reflect the pulsatile characteristics of hypothalamic LHRH secretion, and it is generally accepted that the pulse frequency of LH secretion is an index of the frequency of endogenous LHRH pulsation, we used LH pulse frequency as the indicator of LHRH pulse frequency. Frequent blood sampling was performed to characterize LH pulse patterns in five men with selective elevations of FSH and seven age-matched normal men. Beginning at 0800-0930 h, blood samples were obtained every 10 min for 24 h through an indwelling iv catheter. Serum LH and FSH levels were measured by RIA in each sample, and the pattern of LH secretion was determined. Testosterone (T), estradiol, sex hormone-binding globulin, and free T were measured in a pooled serum sample from each man. Men with selective elevations of FSH had fewer LH pulses per 24 h (mean +/- SEM, 10.6 +/- 0.5) than the control group (12.9 +/- 0.6; P less than 0.01). There was no statistically significant difference in LH pulse amplitude (23 +/- 4 vs. 17 +/- 3 ng/ml). There were no statistically significant differences in T (4.9 +/- 0.5 vs. 6.1 +/- 0.5 ng/ml), estradiol (23 +/- 7 vs. 31 +/- 5 pg/ml), sex hormone-binding globulin (7.7 +/- 1.4 vs. 7.7 +/- 1.2 ng bound dihydrotestosterone/ml), or free T (0.16 +/- 0.02 vs. 0.23 +/- 0.04 ng/ml) in these men vs. normal subjects. We conclude that 1) compared to normal men, men with selectively elevated FSH levels have decreased LH pulse frequency, which suggests decreased LHRH pulse frequency; and 2) the relative secretion rates of LH and FSH by the pituitary may be regulated by the frequency of pulsatile LHRH secretion from the hypothalamus.
为检验内源性脉冲式促黄体生成素释放激素（LHRH）刺激频率控制垂体中促卵泡激素（FSH）和促黄体生成素（LH）相对分泌这一假说，我们研究了FSH水平升高而LH水平正常的男性，以确定与正常男性相比，他们的脉冲式LHRH分泌频率是否改变。由于外周血中LHRH的测量不能反映下丘脑LHRH分泌的脉冲特征，且一般认为LH分泌的脉冲频率是内源性LHRH脉冲频率的指标，所以我们用LH脉冲频率作为LHRH脉冲频率的指标。对5名FSH选择性升高的男性和7名年龄匹配的正常男性进行频繁采血，以描绘LH脉冲模式。从0800 - 0930时开始，通过留置静脉导管每10分钟采集一次血样，持续24小时。用放射免疫分析法（RIA）测定每个样本中的血清LH和FSH水平，并确定LH分泌模式。测定每个男性混合血清样本中的睾酮（T）、雌二醇、性激素结合球蛋白和游离T。FSH选择性升高的男性每24小时的LH脉冲次数（平均值±标准误，10.6±0.5）少于对照组（12.9±0.6；P＜0.01）。LH脉冲幅度无统计学显著差异（23±4对17±3 ng/ml）。这些男性与正常受试者相比，T（4.9±0.5对6.1±0.5 ng/ml）、雌二醇（23±7对31±5 pg/ml）、性激素结合球蛋白（7.7±1.4对7.7±1.2 ng结合双氢睾酮/ml）或游离T（0.16±0.02对0.23±0.04 ng/ml）均无统计学显著差异。我们得出结论：1）与正常男性相比，FSH水平选择性升高的男性LH脉冲频率降低，这表明LHRH脉冲频率降低；2）垂体中LH和FSH的相对分泌率可能受下丘脑脉冲式LHRH分泌频率的调节。
