Laboratório de Estudos Ornitológicos (Labeo), Faculdade de Veterinária (FAVET), Universidade Estadual do Ceará (UECE), Brazil.
OnCells Biotechnology, Brazil.
Comp Immunol Microbiol Infect Dis. 2024 Sep;112:102227. doi: 10.1016/j.cimid.2024.102227. Epub 2024 Aug 8.
The current study aimed to detect virulence, hetero-pathogenicity, and hybridization genes in Escherichia coli strains, previously isolated from cloacal swabs in commercial breeding psittacines and zoological collections, via multiplex PCR. A total of 68 strains of E. coli, previously isolated from psittacines in zoos and commercial breeding facilities in Ceará, Brazil, were assessed for the presence of the following genes and/or probes: eae, bfpA (EPEC - Enteropathogenic E. coli), CVD432 (EAEC - Enteroaggregative E. coli); LT gene and ST gene (ETEC - Enterotoxigenic E. coli); ipaH (EIEC - Enteroinvasive E. coli); stx1 and stx2 (STEC - Shiga toxin-producing E. coli); iroN, ompT, hlyF, iss, and iutA (APEC - Avian pathogenic E. coli). Of the 68 E. coli strains analyzed, 61 (98.7 %) were positive for the following genes and/or probes: Stx1 (61/98.7 %), ST gene (54/79.4 %), CVD432 (49/72 %), bfpA (44/64.7 %), eae (42/61.8 %), Stx2 (41/60.3 %), ipaH (34/50 %), LT gene (33/48.5 %), iroN (21/30.9 %), hlyF (11/6.2 %), iss (06/8.8 %) and iutA (06/8.8 %). The following diarrheagenic pathotypes were identified: 66 (97 %) from STEC, 49 (72 %) from EAEC, 35 (52 %) from EIEC, 25 (37 %) from ETEC, and one (1.5 %) from EPEC. Regarding hetero-pathogenicity, 50 (74 %) heterogeneous strains were identified. Positivity for APEC was seen in four (6 %) strains, all characterized as pathogenic hybrids. This study describes significant associations of virulence factors in E. coli strains DEC/DEC and DEC/APEC, which were isolated from psittacines and may be potentially harmful to One Health.
本研究旨在通过多重 PCR 检测先前从巴西塞阿拉州商业养殖鹦鹉和动物园禽类的泄殖腔拭子中分离出的大肠杆菌菌株的毒力、异致病性和杂交基因。巴西塞阿拉州动物园和商业养殖设施中分离出的 68 株大肠杆菌，评估了以下基因和/或探针的存在情况：eae、bfpA（EPEC-肠致病性大肠杆菌）、CVD432（EAEC-肠聚集性大肠杆菌）；LT 基因和 ST 基因（ETEC-肠毒性大肠杆菌）；ipaH（EIEC-肠侵袭性大肠杆菌）；stx1 和 stx2（STEC-产志贺毒素大肠杆菌）；iroN、ompT、hlyF、iss 和 iutA（APEC-禽致病性大肠杆菌）。在分析的 68 株大肠杆菌中，61 株（98.7%）为以下基因和/或探针阳性：Stx1（61/98.7%）、ST 基因（54/79.4%）、CVD432（49/72%）、bfpA（44/64.7%）、eae（42/61.8%）、Stx2（41/60.3%）、ipaH（34/50%）、LT 基因（33/48.5%）、iroN（21/30.9%）、hlyF（11/6.2%）、iss（06/8.8%）和 iutA（06/8.8%）。鉴定出以下 6 种腹泻性病原体：66 株（97%）来自 STEC、49 株（72%）来自 EAEC、35 株（52%）来自 EIEC、25 株（37%）来自 ETEC、1 株（1.5%）来自 EPEC。关于异致病性，鉴定出 50 株（74%）异质菌株。4 株（6%）被鉴定为致病性杂交株的分离株呈 APEC 阳性。本研究描述了在 DEC/DEC 和 DEC/APEC 大肠杆菌分离株中发现的毒力因子的显著相关性，这些分离株来自鹦鹉，可能对人类健康构成潜在威胁。
