Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz 71441-65186, Iran.
Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz 71441-65186, Iran.
Food Chem. 2024 Dec 15;461:140856. doi: 10.1016/j.foodchem.2024.140856. Epub 2024 Aug 13.
A rapid user-friendly paper-based test strip using zinc microparticles in conjugation with Griess reagent was developed for nitrite and nitrate detection. Test strips were fabricated using a simple and fast method of step-by-step immersion into reagents so that each strip contained a single detection pad for nitrite detection and another separate pad for nitrate detection. To reduce nitrate to nitrite, zinc microparticles suspended in ethanolic solution of polyvinylpyrrolidone (PVP) were uniformly immobilized on the paper strips that were previously impregnated in the Griess reagent and dried. The Griess reagent components were optimized to reach the highest color intensity. The optimized test strip was able to determine both nitrite and nitrate with respective detection limits of 0.43 and 9.43 mg/kg and a detection time of 60 s. The performance of the new test strips was evaluated for the simultaneous colorimetric detection of nitrite and nitrate in water and different food samples.
一种快速易用的基于纸张的测试条，使用锌微粒与格里斯试剂结合，用于亚硝酸盐和硝酸盐的检测。测试条是使用简单快速的分步浸入试剂的方法制造的，因此每条测试条都包含一个用于检测亚硝酸盐的单个检测垫和另一个用于检测硝酸盐的单独垫。为了将硝酸盐还原为亚硝酸盐，悬浮在聚乙烯吡咯烷酮（PVP）乙醇溶液中的锌微粒被均匀地固定在先前浸渍在格里斯试剂并干燥的纸条上。优化了格里斯试剂成分以达到最高的颜色强度。优化后的测试条能够分别检测到亚硝酸盐和硝酸盐，检测限分别为 0.43 和 9.43mg/kg，检测时间为 60s。新测试条的性能用于同时比色检测水和不同食品样品中的亚硝酸盐和硝酸盐。
