Coll Food Sci & Light Ind, Nanjing Tech University, Nanjing 211800, China.
Coll Food Sci & Light Ind, Nanjing Tech University, Nanjing 211800, China.
Food Chem. 2024 Dec 15;461:140842. doi: 10.1016/j.foodchem.2024.140842. Epub 2024 Aug 10.
A GPE-PET (graphene-polyethylene terephthalate) bipolar electrode-electrochemiluminescence (BPE-ECL) platform was developed for ochratoxin A (OTA) detection. PET served as the electrode sheet substrate, and GPE was drop-coated onto the surface of PET to form a conductive line. On the functional sensing interface, the thiol (-SH) modified OTA aptamer (OTA-Aptamer) are fixed on the surface of the gold-plated cathode through AuS bonds. The efficient electron transfer ability of methylene blue (MB) made the anode ECL signal strong. Due to competition between OTA and MB with OTA-Aptamer, leading to a decrease in ECL intensity of the [Ru(bpy)]/TPA system on the BPE anode. Under optimized conditions, the GPE-PET BPE-ECL biosensor displayed superior sensitivity for OTA with a detection limit of 2 ng mL and a wide linear concentration range of 5-100 ng mL. This method could be further applied to detect various toxins and had broad application prospects.
一种 GPE-PET（石墨烯-聚对苯二甲酸乙二醇酯）双极电极-电化学发光（BPE-ECL）平台被开发用于检测赭曲霉毒素 A（OTA）。PET 用作电极片基底，GPE 滴涂在 PET 表面上形成导电线。在功能传感界面上，巯基（-SH）修饰的 OTA 适体（OTA-Aptamer）通过 AuS 键固定在镀金阴极的表面上。亚甲基蓝（MB）具有高效的电子转移能力，使阳极 ECL 信号较强。由于 OTA 与 OTA-Aptamer 与 MB 之间的竞争，导致 BPE 阳极上 [Ru（bpy）] / TPA 体系的 ECL 强度降低。在优化条件下，GPE-PET BPE-ECL 生物传感器对 OTA 具有优异的灵敏度，检测限为 2 ng mL，线性浓度范围为 5-100 ng mL。该方法可进一步用于检测各种毒素，具有广阔的应用前景。
