The department of Chemistry and Environment Science, Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China.
The department of Chemistry and Environment Science, Fujian Provincial Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China.
Food Chem. 2024 Dec 15;461:140906. doi: 10.1016/j.foodchem.2024.140906. Epub 2024 Aug 21.
A novel sensitive and reusable electrochemical biosensor for Listeria monocytegenes DNA has been constructed based on the recognition of water-soluble hydroxylated fullerene (fullerol) to single- and double-stranded DNA. First, the fullerol was electrodeposited on glassy carbon electrode (GCE), acting as a matrix for non-covalent adsorption of single-stranded probe DNA. Upon hybridization with the target DNA, the double helix structure was formed and desorbed from the electrode surface, driving synchronous regeneration of the biosensing interfaces. The biosensor showed a probe DNA loading density of 144 pmol∙cm with the hybridization efficiency of 72.2%. The biosensor is applicable for the analysis of target DNA in actual milk samples with recoveries between 101.0% and 104.0%. This sensing platform provides a simple method for the construction of sensitive and reusable biosensor to monitor Listeria monocytogenes-related food pollution.
基于水溶性羟基化富勒烯（富勒醇）对单链和双链 DNA 的识别，构建了一种用于单核细胞增生李斯特菌 DNA 的新型敏感且可重复使用的电化学生物传感器。首先，富勒醇被电化学沉积在玻碳电极（GCE）上，作为单链探针 DNA 非共价吸附的基质。与靶 DNA 杂交后，形成双链结构并从电极表面解吸，从而驱动生物传感界面的同步再生。该生物传感器的探针 DNA 加载密度为 144 pmol·cm，杂交效率为 72.2%。该生物传感器适用于实际牛奶样品中目标 DNA 的分析，回收率在 101.0%到 104.0%之间。该传感平台为构建用于监测李斯特菌相关食物污染的敏感且可重复使用的生物传感器提供了一种简单的方法。
