CgIκB2 通过 Rel/NF-κB 信号负调控栉孔扇贝干扰素样蛋白的表达。
CgIκB2 negatively regulates the expression of interferon-like protein by Rel/NF-κB signal in Crassostrea gigas.
机构信息
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, 116023, China; Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, 116023, China.
出版信息
Fish Shellfish Immunol. 2024 Oct;153:109853. doi: 10.1016/j.fsi.2024.109853. Epub 2024 Aug 21.
Inhibitors of NF-κB (IκBs) have been implicated as major components of the Rel/NF-κB signaling pathway, playing an important negative regulatory role in host antiviral immunity such as in the activation of interferon (IFN) in vertebrates. In the present study, the immunomodulatory effect of IκB (CgIκB2) on the expression of interferon-like protein (CgIFNLP) was evaluated in Pacific oyster (Crassostrea gigas). After poly (I:C) stimulation, the mRNA expression level of CgIκB2 in haemocytes was significantly down-regulated at 3-12 h while up-regulated at 48-72 h. The mRNA expression of CgIκB2 in haemocytes was significantly up-regulated at 3 h after rCgIFNLP stimulation. In the CgIκB2-RNAi oysters, the mRNA expression of CgIFNLP, interferon regulatory factor-8 (CgIRF8) and NF-κB subunit (CgRel), the abundance of CgIFNLP and CgIRF8 protein in haemocytes, as well as the abundance of CgRel protein in nucleus were significantly increased after poly (I:C) stimulation. Immunofluorescence assay showed that nuclear translocation of CgIRF8 and CgRel protein was promoted in CgIκB2-RNAi oysters compared with that in EGFP-RNAi group. In the CgRel-RNAi oysters, the mRNA and protein expression level of CgIFNLP significantly down-regulated after poly (I:C) stimulation. The collective results indicated that CgIκB2 plays an important role in regulating CgIFNLP expression through its effects on Rel/NF-κB and IRF signaling pathways.
NF-κB（IκB）抑制剂被认为是 Rel/NF-κB 信号通路的主要组成部分，在脊椎动物的干扰素（IFN）激活等宿主抗病毒免疫中发挥重要的负调控作用。本研究评估了 IκB（CgIκB2）对太平洋牡蛎（Crassostrea gigas）干扰素样蛋白（CgIFNLP）表达的免疫调节作用。在多聚（I:C）刺激后，血细胞中 CgIκB2 的 mRNA 表达水平在 3-12 小时显著下调，而在 48-72 小时显著上调。rCgIFNLP 刺激后 3 小时血细胞中 CgIκB2 的 mRNA 表达显著上调。在 CgIκB2-RNAi 牡蛎中，多聚（I:C）刺激后 CgIFNLP、干扰素调节因子 8（CgIRF8）和 NF-κB 亚基（CgRel）的 mRNA 表达、CgIFNLP 和 CgIRF8 蛋白在血细胞中的丰度以及 CgRel 蛋白在核中的丰度均显著增加。免疫荧光分析显示，与 EGFP-RNAi 组相比，CgIκB2-RNAi 牡蛎中 CgIRF8 和 CgRel 蛋白的核转位增加。在 CgRel-RNAi 牡蛎中，多聚（I:C）刺激后 CgIFNLP 的 mRNA 和蛋白表达水平显著下调。综上所述，CgIκB2 通过其对 Rel/NF-κB 和 IRF 信号通路的影响，在调节 CgIFNLP 表达中发挥重要作用。
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