School of Optometry and Vision Science, Faculty of Science, The University of New South Wales, Sydney, New South Wales, Australia; Dougenzaka Itoi Eye Clinic, Itoi Building 1-10-19 Dougenzaka Shibuya-ku, Tokyo, Japan.
School of Optometry and Vision Science, Faculty of Science, The University of New South Wales, Sydney, New South Wales, Australia.
Cont Lens Anterior Eye. 2024 Dec;47(6):102288. doi: 10.1016/j.clae.2024.102288. Epub 2024 Aug 22.
This study aimed to assess the susceptibility of ocular isolates of Pseudomonas aeruginosa to two multi-purpose disinfectant solutions (MPDS) and to determine if there was a relationship with resistance to antibiotics.
Twenty-three strains of P. aeruginosa isolated from microbial keratitis cases in Australia were utilized in this study. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of two commercially available MPDSs, Biotrue and cleadew MPS were determined. Additionally, the MIC of the strains to five antibiotics, ciprofloxacin, levofloxacin, gentamicin, ceftazidime, and imipenem, were analyzed.
All strains were susceptible to 100 % of each of the MPDS. However, when MPDSs were diluted, cleadew MPS had significantly lower median MIC (median = 12.5 vs 25; p = 0.00008) and MBC (median = 25 vs 50; p = 0.0027) compared to Biotrue. All tested strains were susceptible to levofloxacin and gentamicin. Susceptibility rates to ciprofloxacin, imipenem, and ceftazidime were 52.2 %, 30.4 %, and 91.3 %, respectively. There were no significant relations between MIC or MBC to either MPDS and resistance to the antibiotics (p≧0.23).
Both MPDSs were active against P. aeruginosa isolated from microbial keratitis in Australia. However, after dilution, cleadew MPS remained active against P. aeruginosa at lower concentrations. Certain strains of P. aeruginosa were resistant to imipenem, ceftazidime or ciprofloxacin. The lack of association of susceptibility to MPDS and antibiotics suggest that resistance to one did not predispose to resistance to the other.
本研究旨在评估两种多用途消毒剂（MPDS）对绿脓假单胞菌眼部分离株的敏感性，并确定其与抗生素耐药性之间是否存在关系。
本研究使用了 23 株从澳大利亚微生物角膜炎病例中分离出的绿脓假单胞菌。测定了两种市售 MPDS（Biotrue 和 cleadew MPS）的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。此外，还分析了这些菌株对五种抗生素（环丙沙星、左氧氟沙星、庆大霉素、头孢他啶和亚胺培南）的 MIC。
所有菌株均对两种 MPDS 的 100%有效。然而，当 MPDS 稀释时，cleadew MPS 的 MIC（中位数=12.5 比 25；p=0.00008）和 MBC（中位数=25 比 50；p=0.0027）明显低于 Biotrue。所有测试菌株对左氧氟沙星和庆大霉素均敏感。对环丙沙星、亚胺培南和头孢他啶的敏感性率分别为 52.2%、30.4%和 91.3%。两种 MPDS 的 MIC 或 MBC 与抗生素耐药性之间无显著关系（p≧0.23）。
两种 MPDS 对澳大利亚微生物角膜炎分离出的绿脓假单胞菌均有效。然而，稀释后，cleadew MPS 仍能以较低浓度有效对抗绿脓假单胞菌。某些绿脓假单胞菌菌株对亚胺培南、头孢他啶或环丙沙星耐药。对 MPDS 和抗生素的敏感性缺乏关联表明，对一种药物的耐药性不会导致对另一种药物的耐药性。
