Zhang Shaolun, Gao Cong, Li Xiaomin, Liu Jia, Chen Xiulai, Liu Liming
Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2386-2402. doi: 10.13345/j.cjb.240127.
1, 3-propanediol is an important monomer for the production of polytrimethylene terephthalate (PTT). Currently, it is mainly produced by microbial fermentation, which, however, has low production efficiency. To address this problem, this study employed atmospheric room temperature plasma (ARTP) mutagenesis technology and high-throughput screening to obtain a strain with high tolerance to osmotic pressure, which achieved a 1, 3-propanediol titer of 87 g/L. Furthermore, the gene expression elements suitable for were screened, and metabolic engineering was employed to block redundant metabolic pathways (deletion of , , and ) and enhance the synthesis pathway (overexpression of and ). The titer of 1, 3-propanediol produced by the engineered strain increased to 107 g/L. Finally, in a 5 L fermenter, the optimal strain KP-FMME-6 achieved a 1, 3-propanediol titer of 118 g/L, with a glycerol conversion rate of 42% and productivity of 2.46 g/(h·L), after optimization of the fermentation parameters. This study provides a reference for the industrial production of 1, 3-propanediol.
1,3 - 丙二醇是生产聚对苯二甲酸丙二醇酯（PTT）的重要单体。目前，它主要通过微生物发酵生产，然而，这种方法生产效率较低。为了解决这个问题，本研究采用常压室温等离子体（ARTP）诱变技术和高通量筛选，获得了一株具有高渗透压耐受性的菌株，该菌株的1,3 - 丙二醇产量达到87 g/L。此外，筛选了适合的基因表达元件，并采用代谢工程阻断冗余代谢途径（删除 、 和 ），增强合成途径（过表达 和 ）。工程菌株生产的1,3 - 丙二醇产量提高到107 g/L。最后，在5 L发酵罐中，经过发酵参数优化，最优菌株KP - FMME - 6的1,3 - 丙二醇产量达到118 g/L，甘油转化率为42%，生产效率为2.46 g/(h·L)。本研究为1,3 - 丙二醇的工业化生产提供了参考。
