Amna Bibi, Su Liqiu, Dai Zongjie, Wang Qinhong
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Aug 25;40(8):2473-2488. doi: 10.13345/j.cjb.240165.
Terpenoids, known for their structural and functional diversity, are highly valued, especially in food, cosmetics, and cleaning products. Microbial biosynthesis has emerged as a sustainable and environmentally friendly approach for the production of terpenoids. However, the natural enzymes involved in the synthesis of terpenoids have problems such as low activity, poor specificity, and insufficient stability, which limit the biosynthesis efficiency. Enzyme engineering plays a pivotal role in the microbial synthesis of terpenoids. By modifying the structures and functions of key enzymes, researchers have significantly improved the catalytic activity, specificity, and stability of enzymes related to terpenoid synthesis, providing strong support for the sustainable production of terpenoids. This article reviews the strategies for the modification of key enzymes in microbial synthesis of terpenoids, including improving enzyme activity and stability, changing specificity, and promoting mass transfer through multi-enzyme collaboration. Additionally, this article looks forward to the challenges and development directions of enzyme engineering in the microbial synthesis of terpenoids.
萜类化合物以其结构和功能的多样性而闻名，具有很高的价值，尤其是在食品、化妆品和清洁产品中。微生物生物合成已成为一种可持续且环保的萜类化合物生产方法。然而，参与萜类化合物合成的天然酶存在活性低、特异性差和稳定性不足等问题，这限制了生物合成效率。酶工程在萜类化合物的微生物合成中起着关键作用。通过修饰关键酶的结构和功能，研究人员显著提高了与萜类化合物合成相关酶的催化活性、特异性和稳定性，为萜类化合物的可持续生产提供了有力支持。本文综述了萜类化合物微生物合成中关键酶的修饰策略，包括提高酶活性和稳定性、改变特异性以及通过多酶协作促进传质。此外，本文还展望了酶工程在萜类化合物微生物合成中的挑战和发展方向。
