College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
J Agric Food Chem. 2024 Sep 4;72(35):19353-19365. doi: 10.1021/acs.jafc.4c03554. Epub 2024 Aug 22.
Based on digestion, micellar synthesis, and Caco-2 cell model, this study investigated the effects of typical flavonoids in citrus (naringenin, naringin, hesperetin, hesperidin, quercetin, and rutin) at different doses on the micellization and cellular uptake of -carotene. In digestion, low-dose flavonoids enhanced -carotene bioaccesssibility by regulating the stability and dispersibility of the intestinal medium, particularly quercetin, which significantly increased the bioaccessibility by 44.6% ( < 0.05). Furthermore, naringenin, hesperetin, hesperidin, and quercetin enhanced the micellar incorporation rate of -carotene; however, naringin and rutin exhibited an opposite effect, particularly naringin, which significantly reduced it by 71.3% ( < 0.05). This phenomenon could be attributed to the high solubility of naringin and rutin in micelles, resulting in a competitive inhibitory effect on -carotene. Besides, all treatments significantly enhanced -carotene cellular uptake ( < 0.05) by promoting the expression of scavenger receptor class B type I and Niemann-Pick C1-Like 1.
基于消化、胶束合成和 Caco-2 细胞模型，本研究探讨了柑橘类典型类黄酮（柚皮苷、橙皮苷、柚皮素、橙皮素、槲皮素和芦丁）在不同剂量下对 -胡萝卜素胶束化和细胞摄取的影响。在消化过程中，低剂量的类黄酮通过调节肠道介质的稳定性和分散性来提高 -胡萝卜素的生物利用度，特别是槲皮素，其生物利用度显著提高了 44.6%（<0.05）。此外，柚皮苷、柚皮素、橙皮素和槲皮素均能提高 -胡萝卜素的胶束化掺入率；然而，橙皮苷和芦丁则表现出相反的效果，特别是橙皮苷，其显著降低了 71.3%（<0.05）。这种现象可能归因于橙皮苷和芦丁在胶束中的高溶解度，导致对 -胡萝卜素的竞争性抑制作用。此外，所有处理均通过促进清道夫受体 B 类 I 和 Niemann-Pick C1 样 1 的表达显著提高了 -胡萝卜素的细胞摄取（<0.05）。
