Kikutani H, Taga T, Akira S, Kishi H, Miki Y, Saiki O, Yamamura Y, Kishimoto T
J Immunol. 1985 Feb;134(2):990-5.
Stimulation of human B lymphoblastoid cell lines, CESS and SKW6-CL4 with BCDF induced an increase in IgG- and IgM-secreting cells, as well as a slight increase in IgG and IgM expression on their respective surfaces. Biosynthetic labeling demonstrated that BCDF stimulation induced an increase in synthesis of secretory gamma- and mu-chains, as well as their precursors. A slight but significant increase in synthesis of membrane-bound gamma- and mu-chains and their precursors was also observed by BCDF stimulation. However, an increase in synthesis of secretory heavy chains was much higher than that in membrane-bound heavy chains in both cell lines. Pulse-chase experiments showed that increased synthesis of secretory heavy chains was not due to a decrease in degradation. BCDF stimulation induced a preferential increase in mRNA specific for secretory gamma- and mu-chains in CESS and SKW6-CL4 cells, respectively. These results suggest that BCDF induces an increase in the level of mRNA specific for secretory heavy chains, and then induces final maturation of B cells into immunoglobulin-secreting cells.
用BCDF刺激人B淋巴母细胞系CESS和SKW6 - CL4，可诱导IgG和IgM分泌细胞增加，以及它们各自表面IgG和IgM表达略有增加。生物合成标记表明，BCDF刺激可诱导分泌性γ链和μ链及其前体的合成增加。BCDF刺激还观察到膜结合γ链和μ链及其前体的合成有轻微但显著的增加。然而，在这两种细胞系中，分泌性重链的合成增加远高于膜结合重链。脉冲追踪实验表明，分泌性重链合成的增加并非由于降解减少。BCDF刺激分别在CESS和SKW6 - CL4细胞中诱导分泌性γ链和μ链特异性mRNA的优先增加。这些结果表明，BCDF诱导分泌性重链特异性mRNA水平增加，然后诱导B细胞最终成熟为免疫球蛋白分泌细胞。
