Kishi H, Miki Y, Kikutani H, Yamamura Y, Kishimoto T
J Immunol. 1983 Oct;131(4):1961-5.
The incubation of CESS cells with BCDF induced phospholipid methylation, i.e., 3H-methyl incorporation into phosphatidylcholine and phosphatidyl-N-monomethyl-ethanolamine, within 15 to 30 min. A threefold increase of 3H-DFP-binding was also observed in BCDF-stimulated CESS cells 2 hr after stimulation, showing the activation of serine esterase after phospholipid methylation. The inhibitors of methyltransferase, SIBA, adenosine, or HEH showed a dose-dependent inhibitory effect not only on phospholipid methylation but also on serine esterase activation and on BCDF-induced IgG production; DFP inhibited IgG production but not phospholipid methylation. The treatment of cells with DFP in the presence of SIBA abrogated the irreversible inhibitory effect of DFP on IgG production. These results showed that BCDF stimulation induced phospholipid methylation that set the stage for serine esterase activation and IgG induction.
将CESS细胞与BCDF一起温育，在15至30分钟内可诱导磷脂甲基化，即3H-甲基掺入磷脂酰胆碱和磷脂酰-N-单甲基乙醇胺中。在刺激后2小时，在BCDF刺激的CESS细胞中也观察到3H-DFP结合增加了三倍，表明磷脂甲基化后丝氨酸酯酶被激活。甲基转移酶抑制剂SIBA、腺苷或HEH不仅对磷脂甲基化有剂量依赖性抑制作用，而且对丝氨酸酯酶激活和BCDF诱导的IgG产生也有抑制作用；DFP抑制IgG产生，但不抑制磷脂甲基化。在SIBA存在下用DFP处理细胞消除了DFP对IgG产生的不可逆抑制作用。这些结果表明，BCDF刺激诱导了磷脂甲基化，为丝氨酸酯酶激活和IgG诱导奠定了基础。
