Department of Biology, Faculty of Science, Al-Baha University, Al-Baha, Saudi Arabia.
Open Vet J. 2024 Jul;14(7):1568-1576. doi: 10.5455/OVJ.2024.v14.i7.6. Epub 2024 Jul 31.
An ectoparasite known as feeds on infected blood with a high frequency in European chicken farms resulting in significant economic losses.
The objective of the current work was to characterize , which infests laying hens on farms in Southern Al-Baha morphologically, and molecularly, and to determine the evolutionary relationship between the species.
All mites that were morphologically recognized as were submitted to molecular analysis by PCR, which focused on the mitochondrial cytochrome oxidase subunit I and internal transcribed spacers (ITS) of ribosomal DNA.
Morphological identification of the parasites uncovered three distinct features: a triangular anal shield, a broader than longer sternal shield, and a rounded posterior genitoventral shield. Each sample was amplified using a single band, measuring 550 bp for the cox1-targeting PCR, and 530 bp for the ITS-targeting PCR. The sequences of were added to the GenBank.
At the molecular identification level, this research identifies in Al-Baha for the first time. The results collectively provide a foundation for further research to understand the epidemiology and the part of this superfamily in the epidemiology of certain zoonosis.
一种名为 的外寄生虫在欧洲的养鸡场中以高频吸食受感染的血液，导致了巨大的经济损失。
本研究的目的是从形态学和分子学上对感染产蛋鸡的 进行鉴定，并确定该物种之间的进化关系。
所有被形态学识别为 的螨虫都通过聚合酶链反应（PCR）进行了分子分析，该分析集中在线粒体细胞色素氧化酶亚基 I（cox1）和核糖体 DNA 的内部转录间隔区（ITS）上。
寄生虫的形态学鉴定揭示了三个明显的特征：三角形的肛盾、比长更宽的胸盾，以及圆形的后生殖器盾。每个 样本都通过单个带进行了扩增，cox1 靶向 PCR 的扩增产物为 550bp，ITS 靶向 PCR 的扩增产物为 530bp。 的序列已被添加到 GenBank 中。
在分子鉴定水平上，本研究首次在阿巴哈地区鉴定出 。这些结果为进一步研究了解该超家族在某些人畜共患病流行病学中的作用提供了基础。
