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在塔吉克盘羊物种中成功移植精子冷冻、体外受精和人工授精方法（）。

Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species ().

作者信息

Locatelli Yann, Vion Colin, Duffard Nicolas, Bon Nicolas, Bernard Jérémy, Binaud Charly, Trontti Nina, Baril Gérard, Mermillod Pascal

机构信息

Muséum National d'Histoire Naturelle, Laboratoire de la Réserve Zoologique de la Haute Touche, Obterre, France.

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique, Université de Tours, Nouzilly, France.

出版信息

Anim Reprod. 2024 Aug 12;21(3):e20240071. doi: 10.1590/1984-3143-AR2024-0071. eCollection 2024.

DOI:10.1590/1984-3143-AR2024-0071
PMID:39175995
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11340789/
Abstract

The objective of the present study was to transpose sperm freezing methodology from domestic goat to the Tadjik markhor () and to address the feasibility to develop IVP and artificial insemination using such frozen semen. Semen of different adult markhor males were successfully recovered by electro-ejaculation and were then frozen using caprine methodology. Frozen semen showed good survival rates at thawing and good fertility rates were assessed in heterologous fertilization system with goat oocytes. LOPU/IVF was applied for Tadjik markhor females allowing the first successful blastocyst production . In an applied program, we also transposed successfully intrauterine AI method with frozen/thawed semen to the Tadjik markhor.

摘要

本研究的目的是将家山羊的精子冷冻方法应用于塔吉克盘羊，并探讨使用这种冷冻精液开发体外受精（IVP）和人工授精的可行性。通过电刺激采精成功采集了不同成年盘羊雄性的精液，然后采用山羊的方法进行冷冻。冷冻精液解冻后显示出良好的存活率，并且在与山羊卵母细胞的异种受精系统中评估了良好的受精率。对塔吉克盘羊雌性应用腹腔镜采卵/体外受精（LOPU/IVF）技术，首次成功获得了囊胚。在一个应用项目中，我们还成功地将冷冻/解冻精液的子宫内人工授精方法应用于塔吉克盘羊。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e7e/11340789/970ec4eac8a0/1984-3143-ar-21-3-e20240071-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e7e/11340789/5a1281cf8289/1984-3143-ar-21-3-e20240071-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e7e/11340789/970ec4eac8a0/1984-3143-ar-21-3-e20240071-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e7e/11340789/5a1281cf8289/1984-3143-ar-21-3-e20240071-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1e7e/11340789/970ec4eac8a0/1984-3143-ar-21-3-e20240071-gf02.jpg

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Anim Reprod. 2022 Mar 21;19(1):e20210093. doi: 10.1590/1984-3143-AR2021-0093. eCollection 2022.
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Intrinsic quality of goat oocytes already found denuded at collection for in vitro embryo production.在收集时已发现去透明带的山羊卵母细胞的内在质量用于体外胚胎生产。
Theriogenology. 2016 Nov;86(8):1989-98. doi: 10.1016/j.theriogenology.2016.06.021. Epub 2016 Jun 29.
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Sperm characteristics and heterologous in vitro fertilisation capacity of Iberian ibex (Capra pyrenaica) epididymal sperm, frozen in the presence of the enzymatic antioxidant catalase.
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Cryobiology. 2014 Jun;68(3):389-94. doi: 10.1016/j.cryobiol.2014.03.009. Epub 2014 Apr 1.
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In vitro embryo production in goats: Slaughterhouse and laparoscopic ovum pick up-derived oocytes have different kinetics and requirements regarding maturation media.山羊体外胚胎生产：屠宰场和腹腔镜取卵获得的卵母细胞在成熟培养基方面具有不同的动力学和要求。
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