Single nucleotide polymorphism profiles of canine T-cell and null-cell lymphomas.

BACKGROUND

The histopathological classification of T-cell lymphoma (TCL) in humans has distinctive mutational genotyping that suggests different lymphomagenesis. A similar concept is assumed to be observed in dogs with different TCL phenotypes.

OBJECTIVE

This study aimed to identify the previously reported single-nucleotide polymorphisms (SNPs) in both human beings and dogs in canine TCLs and null-cell lymphomas (NCLs) and to design compatible oligonucleotides from each variant based on the multiplex polymerase chain reaction.

METHODS

Genomic DNA was extracted from 68 tumor specimens (62 TCLs and 6 NCLs) and 5 buffy coat samples from dogs with TCL. Four TCL subtypes and NCL were analyzed in 44 SNPs from 21 genes using the MassARRAY.

RESULTS

The greatest incidences of SNPs observed in all TCL subtypes and NCL ware c.1259A > C, c.1275A > G, c.2040 + 200C > G, and c.1024C > T, respectively. Some SNP locations were statistically significant associated with NCL, including p.S75F ( = 0.0003), p.I149N ( = 0.030), p.F37LX ( = 0.012), and p.R583* ( = 0.012).

CONCLUSION

Each TCL histological subtype and NCL are likely to contain distinctive mutational genetic profiles, which might play a role in lymphoma gene-risk factors and might be useful for selecting therapeutic target drugs for each canine patient.