Yamamoto K, Tsuji T, Tarutani O, Osawa T
Biochim Biophys Acta. 1985 Jan 28;838(1):84-91. doi: 10.1016/0304-4165(85)90253-3.
Structures of anionic oligosaccharides released by endo-beta-N-acetylglucosaminidase H from human thyroglobulin (19 S) purified from normal and malignant (papillary carcinoma) thyroid tissues were studied by sequential exoglycosidase digestion and lectin affinity chromatography. It was revealed that sialylated hybrid-type oligosaccharides were present in thyroglobulin from normal thyroid tissue, whereas, in the case of thyroglobulin from malignant thyroid tissue, phosphorylated high-mannose type and hybrid-type oligosaccharides each containing one phosphate group in a diester linkage were present instead of sialylated oligosaccharides. The possible meaning of the phosphorylated oligosaccharides was discussed in relation to the low thyroglobulin content in malignant thyroid tissue.
通过外切糖苷酶顺序消化和凝集素亲和色谱法，研究了从正常和恶性（乳头状癌）甲状腺组织中纯化的人甲状腺球蛋白（19S）经内切β-N-乙酰氨基葡萄糖苷酶H释放的阴离子寡糖的结构。结果表明，正常甲状腺组织的甲状腺球蛋白中存在唾液酸化杂合型寡糖，而恶性甲状腺组织的甲状腺球蛋白中则存在磷酸化高甘露糖型和杂合型寡糖，每种寡糖在二酯键中含有一个磷酸基团，而不是唾液酸化寡糖。结合恶性甲状腺组织中甲状腺球蛋白含量低的情况，讨论了磷酸化寡糖的可能意义。
