Grünwald J, Hesz A, Robenek H, Brücker J, Buddecke E
Exp Mol Pathol. 1985 Feb;42(1):60-70. doi: 10.1016/0014-4800(85)90018-8.
Aortic endothelial cells from control and streptozotocin diabetic minipigs were cultured. Both groups of cells exhibited the typical cobblestone-like appearance and gap junction formation. Endothelial cells derived from diabetic minipigs differed, however, from those from control animals by a higher rate of proliferation and a higher percentage of large and often multinucleated cells. In these cells the specific binding of low-density lipoproteins (LDL) to coated pits on the cell surface, the LDL uptake, and the intracellular transport of LDL to lysosomes were visualized by gold-labeled LDL complexes. The binding, internalization, and degradation of LDL by subconfluent, non-contact-inhibited endothelial cells was quantified using 125I-labeled LDL. The LDL metabolism of endothelial cells derived from diabetic animals was increased by about 40% compared to endothelial cells derived from nondiabetic animals.
培养了来自对照和链脲佐菌素诱导的糖尿病小型猪的主动脉内皮细胞。两组细胞均呈现典型的鹅卵石样外观并形成缝隙连接。然而，糖尿病小型猪来源的内皮细胞与对照动物来源的内皮细胞不同，其增殖率更高，且大的、通常为多核的细胞百分比更高。在这些细胞中，通过金标记的低密度脂蛋白（LDL）复合物观察到LDL与细胞表面被膜小窝的特异性结合、LDL摄取以及LDL向溶酶体的细胞内转运。使用125I标记的LDL对亚汇合、非接触抑制的内皮细胞摄取、内化和降解LDL进行定量。与非糖尿病动物来源的内皮细胞相比，糖尿病动物来源的内皮细胞的LDL代谢增加了约40%。
