Plachot M, Mandelbaum J, Cohen J J, Debache C, Pigeau F, Junca A M
Fertil Steril. 1985 Feb;43(2):255-62. doi: 10.1016/s0015-0282(16)48382-2.
Sequential treatment with clomiphene citrate, human menopausal gonadotropin, and human chorionic gonadotropin was successfully applied to 149 women in the framework of an in vitro fertilization and embryo transfer program and led to the birth of normal children. An average of 3.5 follicles was promoted at each cycle. Oocyte maturity was evaluated at recovery either histologically or with respect to cumulus-oocyte complexes (COC) and correlated to oocyte fertilizability. Three types of COC are described according to cell dissociation and mucification. The most expanded cumulus mass (type I) was highly fertilizable, compared with granular and poorly dissociated cumulus complexes (types II and III, respectively). No improvement in the type II and III oocyte fertilizability was obtained when type I follicular fluid was added to the incubation medium. Moreover, incubation of type III oocytes in medium containing type III follicular fluid seemed to decrease their fertilizability.
在体外受精和胚胎移植项目中，对149名女性成功应用了枸橼酸氯米芬、人绝经期促性腺激素和人绒毛膜促性腺激素的序贯治疗，并产下了正常婴儿。每个周期平均促排卵3.5个卵泡。在回收时，通过组织学或根据卵丘-卵母细胞复合体（COC）评估卵母细胞成熟度，并与卵母细胞受精能力相关联。根据细胞解离和黏液形成描述了三种类型的COC。与颗粒状和解离不良的卵丘复合体（分别为II型和III型）相比，扩张最明显的卵丘团块（I型）具有很高的受精能力。当向孵育培养基中添加I型卵泡液时，II型和III型卵母细胞的受精能力没有改善。此外，将III型卵母细胞在含有III型卵泡液的培养基中孵育似乎会降低其受精能力。
