Key Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Institute of Agro-products Storage and Processing & Xinjiang Key Laboratory of Processing and Preservation of Agricultural Products, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, China.
Food Chem. 2024 Dec 15;461:140942. doi: 10.1016/j.foodchem.2024.140942. Epub 2024 Aug 20.
Methionine (Met) can inhibit plant diseases caused by phytopathogens. However, the effect of Met on gray mold resulted from Botrytis cinerea in tomato is still unclear. This study showed 5 mM Met alleviated disease development of gray mold, enhanced chitinase (CHI) and β-1, 3-glucanase (GNS) activities and the expression of SlCHI, SlGNS, SlPR1 and SlNPR1 in tomatoes, rather than inhibited the growth of B. cinerea directly. Moreover, ethylene biosynthesis and signal transduction before pathogen inoculating were induced by 5 mM Met. Interestingly, Met reduced the nitrosylation levels of ACS4 and ACO6, enhanced the activities of nitric oxide synthase, nitrite reductase (NR) and S-nitrosoglutathione reductase (GSNOR) and the expression of SlNR and SlGSNOR. Tomatoes treated with aminoethoxyvinylglycine and carboxy-PTIO exhibited lower resistance to B. cinerea. These results indicate 5 mM Met promoted ethylene biosynthesis and signal transduction to facilitate NO synthesis and metabolism, enhancing the resistance of tomatoes to B. cinerea.
蛋氨酸（Met）可以抑制植物病原菌引起的植物病害。然而，Met 对番茄灰霉病（由 Botrytis cinerea 引起）的影响尚不清楚。本研究表明，5mM Met 可缓解番茄灰霉病的发展，增强几丁质酶（CHI）和β-1,3-葡聚糖酶（GNS）的活性以及 SlCHI、SlGNS、SlPR1 和 SlNPR1 的表达，而不是直接抑制 B. cinerea 的生长。此外，5mM Met 诱导了病菌接种前的乙烯生物合成和信号转导。有趣的是，Met 降低了 ACS4 和 ACO6 的硝化水平，增强了一氧化氮合酶、亚硝酸盐还原酶（NR）和 S-亚硝基谷胱甘肽还原酶（GSNOR）的活性以及 SlNR 和 SlGSNOR 的表达。用氨基乙氧基乙烯基甘氨酸和羧基-PTIO 处理的番茄对 B. cinerea 的抗性较低。这些结果表明，5mM Met 促进了乙烯的生物合成和信号转导，从而促进了 NO 的合成和代谢，增强了番茄对 B. cinerea 的抗性。
