信号识别颗粒 14 在肝细胞癌中的表达及其与疾病进展和患者生存的关系。
Expression of signal recognition particle 14 in hepatocellular carcinoma and its relationship with disease progression and patient survival.
机构信息
Department of Endocrinology and Metabolism, Center for Diabetes and Metabolism Research, West China Hospital, Sichuan University, Chengdu 610041, China.
Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu 610041, China.
出版信息
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2024 Aug 25;53(4):460-471. doi: 10.3724/zdxbyxb-2024-0055.
OBJECTIVES
To investigate the expression of signal recognition particle 14 (SRP14) in hepatocellular carcinoma (HCC) and its clinical significance.
METHODS
The data of SRP14 expression in HCC were obtained from bioinformatics study, and from investigation with quantitative reverse transcription polymerase chain reaction (qRT-PCR), immunohistochemical staining and Western blotting in clinical samples. The Kaplan-Meier analysis was used to determine the associations between mRNA expression and the overall survival, progression-free survival, and disease-specific survival of HCC patients. The effect of SRP14 on the proliferation and migration of HCC cells were determined by EdU staining, MTS, Transwell and wound-healing assays. The potential mechanism for SRP14 regulating HCC was explored through Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analysis as well as qRT-PCR.
RESULTS
According to the data from GSE14520, TNMplot database and clinical samples, compared with paired tumor-adjacent tissues, non-paired tumor-adjacent tissues and normal tissues, the mRNA expression of in HCC tissues was upregulated (all <0.05). In clinical samples, compared with paired tumor-adjacent tissues, the protein expression of SPR14 in HCC tissues was increased (<0.05). The increased mRNA expression of was associated with good overall survival, progression-free survival, and disease-specific survival in HCC patients. SRP14 inhibited the proliferation and migration of HCC cells . According to the KEGG and GO enrichment analysis, in non-specific HCC, the genes co-expressed with SRP14 may predominantly regulate protein synthesis, processing, and transport, while in nonalcoholic fatty liver disease related HCC, the genes co-expressed with SRP14 could control multiple signaling pathways such as MAPK, cAMP, PI3K-Akt, and Wnt. Mechanistically, SRP14 up-regulated the mRNA expression of tumor suppressor gene inHCC cells (<0.05).
CONCLUSIONS
SRP14 may regulate HCC progression and influence patient prognosis.
目的
研究信号识别颗粒 14（SRP14）在肝细胞癌（HCC）中的表达及其临床意义。
方法
从生物信息学研究中获得 HCC 中 SRP14 表达的数据，并从临床样本中进行定量逆转录聚合酶链反应（qRT-PCR）、免疫组织化学染色和 Western blot 分析。Kaplan-Meier 分析用于确定 mRNA 表达与 HCC 患者总生存期、无进展生存期和疾病特异性生存期之间的关联。通过 EdU 染色、MTS、Transwell 和划痕愈合试验测定 SRP14 对 HCC 细胞增殖和迁移的影响。通过京都基因与基因组百科全书（KEGG）和基因本体论（GO）富集分析以及 qRT-PCR 探讨 SRP14 调节 HCC 的潜在机制。
结果
根据 GSE14520、TNMplot 数据库和临床样本的数据，与配对的肿瘤相邻组织、非配对的肿瘤相邻组织和正常组织相比，HCC 组织中 的 mRNA 表达上调（均<0.05）。在临床样本中，与配对的肿瘤相邻组织相比，HCC 组织中 SPR14 的蛋白表达增加（<0.05）。mRNA 表达的增加与 HCC 患者的良好总生存期、无进展生存期和疾病特异性生存期相关。SRP14 抑制 HCC 细胞的增殖和迁移。根据 KEGG 和 GO 富集分析，在非特异性 HCC 中，与 SRP14 共表达的基因可能主要调节蛋白质合成、加工和运输，而在非酒精性脂肪性肝病相关 HCC 中，与 SRP14 共表达的基因可以控制多个信号通路，如 MAPK、cAMP、PI3K-Akt 和 Wnt。在机制上，SRP14 上调 HCC 细胞中肿瘤抑制基因 的 mRNA 表达（<0.05）。
结论
SRP14 可能调节 HCC 的进展并影响患者的预后。
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