Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
The Fifth People's Hospital of Luoyang (The Fifth Affiliated Hospital of Henan University of Science and Technology), No.505 Taikang East Road, Luolong District, Luoyang City, 471000, Henan Province, China.
Sci Rep. 2024 Aug 26;14(1):19735. doi: 10.1038/s41598-024-70258-0.
Meniscus-derived stem cells (MeSCs), a unique type of MSC, have outstanding advantages in meniscal cytotherapy and tissue engineering, but the effects and molecular mechanisms of PBM on MeSCs are still unclear. We used 660-nm LED light with different energy densities to irradiate six human MeSC samples and tested their proliferation rate via cell counting, chondrogenic differentiation capacity via the DMMB assay, mitochondrial activity via the MTT assay, and gene expression via qPCR. The proliferation ability, chondrogenic capacity and mitochondrial activity of the 18 J/cm group were greater than those of the 4 J/cm and control groups. The mRNA expression levels of Akt, PI3K, TGF-β3, Ki67 and Notch-1 in the 18 J/cm group were greater than those in the other groups in most samples. After chondrogenic induction, the expression of Col2A1, Sox9 and Aggrecan in the 18 J/cm group was significantly greater than that in the 4 J/cm and control groups in most of the samples. The variation in the MTT values and Src, PI3K, Akt, mTOR and GSK3β levels decreased with time. The results showed that 660-nm LED red light promoted proliferation and chondrogenic differentiation and affected the gene expression of MeSCs, and the effects on gene expression and mitochondrial activity decreased with time.
半月板来源的干细胞（MeSCs）是一种独特类型的 MSC，在半月板细胞治疗和组织工程中有突出的优势，但 PBM 对 MeSCs 的影响和分子机制尚不清楚。我们使用 660nm LED 光，不同能量密度对 6 个人类 MeSC 样本进行照射，并通过细胞计数测试其增殖率，通过 DMMB 测定测试其软骨分化能力，通过 MTT 测定测试其线粒体活性，通过 qPCR 测试其基因表达。18J/cm 组的增殖能力、软骨分化能力和线粒体活性均大于 4J/cm 组和对照组。在大多数样本中，18J/cm 组的 Akt、PI3K、TGF-β3、Ki67 和 Notch-1 的 mRNA 表达水平均大于其他组。经软骨诱导后，在大多数样本中，18J/cm 组的 Col2A1、Sox9 和 Aggrecan 表达明显大于 4J/cm 组和对照组。MTT 值和 Src、PI3K、Akt、mTOR 和 GSK3β 水平的变化随时间而减少。结果表明，660nm LED 红光促进增殖和软骨分化，并影响 MeSCs 的基因表达，其对基因表达和线粒体活性的影响随时间而减少。
