College of Grassland Science, Inner Mongolia Minzu University, Tongliao, China.
Arch Insect Biochem Physiol. 2024 Aug;116(4):e22145. doi: 10.1002/arch.22145.
Heat shock proteins (Hsp) function as crucial molecular chaperones, playing pivotal roles in insects' response to stress stimuli. Apolygus lucorum, known for its broad spectrum of host plants and significant crop damage potential, presents a compelling subject for understanding stress response mechanisms. Hsp is important for A. lucorum to tolerate temperature and insecticide stress and may be involved in the formation of resistance to the interactive effects of temperature and insecticide. Here, we employed comprehensive genomic approaches to identify Hsp superfamily members in its genome. In total, we identified 42 Hsp genes, including 3 Hsp90, 16 Hsp70, 13 Hsp60, and 10 Hsp20. Notably, we conducted motif analysis and gene structures for Hsp members, which suggested the same families are relatively conserved. Furthermore, leveraging the weighted gene coexpression network analysis, we observed diverse expression patterns of different Hsp types across various tissues, with certain Hsp70 showing tissue-specific bias. Noteworthy among the highly expressed Hsp genes was testis-specific, which may serve as a pivotal hub gene regulating the gene network. Our findings shed light on the molecular evolutionary dynamics and temperature stress response mechanisms of Hsp genes in A. lucorum, offering insights into its adaptive strategies and potential targets for pest management.
热休克蛋白（Hsp）作为重要的分子伴侣，在昆虫应对应激刺激中发挥关键作用。中华婪步甲（Apolygus lucorum）因其广泛的宿主植物和对农作物的巨大破坏潜力而备受关注，是研究应激反应机制的理想对象。Hsp 对中华婪步甲耐受温度和杀虫剂胁迫至关重要，并且可能参与形成对温度和杀虫剂相互作用的抗性。在这里，我们采用全面的基因组方法鉴定了其基因组中的 Hsp 超家族成员。共鉴定出 42 个 Hsp 基因，包括 3 个 Hsp90、16 个 Hsp70、13 个 Hsp60 和 10 个 Hsp20。值得注意的是，我们对 Hsp 成员进行了基序分析和基因结构分析，表明相同的家族相对保守。此外，利用加权基因共表达网络分析，我们观察到不同 Hsp 类型在不同组织中的表达模式存在差异，某些 Hsp70 表现出组织特异性偏好。在高度表达的 Hsp 基因中，睾丸特异性基因尤为突出，可能作为调控基因网络的关键枢纽基因。我们的研究结果揭示了 Hsp 基因在中华婪步甲中的分子进化动态和温度应激响应机制，为其适应策略和害虫管理的潜在靶标提供了新的见解。
