Gizaw Birhanu, Alemu Tesfaye, Ebsa Girma
Department of Microbial, Cellular and Molecular Biology, Addis Ababa University, Addis Ababa, P. O. Box: 1176, Ethiopia.
Heliyon. 2024 Jun 10;10(12):e32769. doi: 10.1016/j.heliyon.2024.e32769. eCollection 2024 Jun 30.
Turquoise blue dye is frequently used for industrial dyeing applications. But the release of untreated colored wastewater became an environmental and public health hazard. Microbial remediation of Azodye is environmentally safe and an alternative to a physicochemical approach. The aim of this research is to isolate and characterize turquoise blue dye degrading microbes from polluted environment. Microbial isolation and purification from soil and effluent sample was done on PDA and NA. Turquoise blue dye degrading test was investigated under optimized conditions using -the definitive screening design method. UV-Vis spectrophotometer used to measure the degradation percentage at 620 nm and 25 °C. The results revealed that 24 fungi and 6 bacterial species were identified from the contaminated site using Biolog Microstation and MALDI-TOF. Among all identified microbial species Thom BCA & show the highest percentage decolorization of turquoise blue dye up to 300 ppm with 90 % removal at pH4 and 87 % at pH 7 up to 400 ppm respectively. The azodye degradation ability of these fungi species used in the development of mycoremediation technologies provide an alternative option for Azodye removal after HPLC analysis, molecular characterization, and toxic analysis.
绿松石蓝染料常用于工业染色应用。但未经处理的有色废水排放成为了环境和公共健康危害。偶氮染料的微生物修复对环境安全，是物理化学方法的一种替代方案。本研究的目的是从受污染环境中分离和鉴定降解绿松石蓝染料的微生物。在马铃薯葡萄糖琼脂（PDA）和营养琼脂（NA）上对土壤和废水样本进行微生物分离和纯化。使用确定性筛选设计方法在优化条件下研究绿松石蓝染料降解试验。用紫外可见分光光度计在620nm波长和25℃下测量降解百分比。结果显示，使用Biolog微生物鉴定系统和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）从污染场地鉴定出24种真菌和6种细菌。在所有鉴定出的微生物物种中，Thom BCA & 在pH4时对高达300ppm的绿松石蓝染料脱色率最高可达90%，在pH7时对高达400ppm的染料脱色率为87%。在进行高效液相色谱（HPLC）分析、分子表征和毒性分析后，这些用于开发真菌修复技术的真菌物种对偶氮染料的降解能力为偶氮染料去除提供了一种替代选择。
