Przystaś Wioletta, Zabłocka-Godlewska Ewa, Grabińska-Sota Elżbieta
Silesian University of Technology, Environmental Biotechnology Department, Gliwice, Poland.
Silesian University of Technology, Environmental Biotechnology Department, Gliwice, Poland.
Braz J Microbiol. 2018 Apr-Jun;49(2):285-295. doi: 10.1016/j.bjm.2017.06.010. Epub 2017 Nov 9.
Different technologies may be used for decolorization of wastewater containing dyes. Among them, biological processes are the most promising because they seem to be environmentally safe. The aim of this study was to determine the efficiency of decolorization of two dyes belonging to different classes (azo and triphenylmethane dyes) by immobilized biomass of strains of fungi (Pleurotus ostreatus - BWPH, Gleophyllum odoratum - DCa and Polyporus picipes - RWP17). Different solid supports were tested for biomass immobilization. The best growth of fungal strains was observed on the washer, brush, grid and sawdust supports. Based on the results of dye adsorption, the brush and the washer were selected for further study. These solid supports adsorbed dyes at a negligible level, while the sawdust adsorbed 82.5% of brilliant green and 19.1% of Evans blue. Immobilization of biomass improved dye removal. Almost complete decolorization of diazo dye Evans blue was reached after 24h in samples of all strains immobilized on the washer. The process was slower when the brush was used for biomass immobilization. Comparable results were reached for brilliant green in samples with biomass of strains BWPH and RWP17. High decolorization effectiveness was reached in samples with dead fungal biomass. Intensive removal of the dyes by biomass immobilized on the washer corresponded to a significant decrease in phytotoxicity and a slight decrease in zootoxicity of the dye solutions. The best decolorization results as well as reduction in toxicity were observed for the strain P. picipes (RWP17).
可采用不同技术对含染料废水进行脱色处理。其中，生物法最具前景，因为它们似乎对环境安全。本研究的目的是确定平菇（Pleurotus ostreatus - BWPH）、香豆胶孔菌（Gleophyllum odoratum - DCa）和毛柄多孔菌（Polyporus picipes - RWP17）等真菌菌株的固定化生物质对两类不同染料（偶氮染料和三苯甲烷染料）的脱色效率。测试了不同的固体载体用于生物质固定化。在垫圈、刷子、网格和锯末载体上观察到真菌菌株的最佳生长情况。基于染料吸附结果，选择刷子和垫圈进行进一步研究。这些固体载体对染料的吸附量可忽略不计，而锯末吸附了82.5%的亮绿和19.1%的伊文思蓝。生物质的固定化提高了染料去除率。固定在垫圈上的所有菌株样品中，偶氮染料伊文思蓝在24小时后几乎完全脱色。当使用刷子进行生物质固定化时，该过程较慢。对于BWPH和RWP17菌株生物质的样品中的亮绿，也得到了类似的结果。在含有死真菌生物质的样品中达到了高脱色效率。固定在垫圈上的生物质对染料的强烈去除对应于染料溶液的植物毒性显著降低和动物毒性略有降低。对于毛柄多孔菌（RWP17）菌株，观察到了最佳的脱色结果以及毒性降低情况。
