Ma Jinnan, Fu Zhaohui, Yang Xin, Ming Wenqin, Song Xuhao, Du Chao
Yunnan Normal University Kunming China.
Baotou Teachers' College Baotou China.
Ecol Evol. 2024 Aug 22;14(8):e70184. doi: 10.1002/ece3.70184. eCollection 2024 Aug.
Insect gut microbiota and their metabolites play a significant role in the shaping of hosts' diets and feeding habits. We conducted 16S rDNA amplicon sequencing on the gut microbiota of specialist blister beetle larvae that feed on locust eggs and artificial food at different instars, to explore the relationship between gut microbiota and the specialized feeding habit of the blister beetle larvae. There is no significant difference in the gut microbial structure among the second to the fourth instar larvae under the same rearing conditions, but the gut microbial structure of the first instar larvae was significantly different from the second to the fourth instar larvae fed by different diets. Bacteria associated with polysaccharide utilization are relatively barren in first instar larvae. Compared to the carbohydrate content between the artificial diet and locust eggs, we speculate that an excessive amount of polysaccharides in the artificial diet may be detrimental to the growth and development of first instar larvae. Gut microbiota of the second to the fourth instar larvae fed with different diets significantly differ in microbial community structure. The different bacteria, especially the metabolism-related intestinal bacteria in locust eggs-fed larvae, may help the hosts adapt to the environment and contribute to the production of active ingredients. The relative abundance of polysaccharide utilization-related bacteria was significantly higher in the artificial diet-fed larvae compared to the locust eggs-fed larvae, which showed the same result when compared to the first instar larvae. Changes in gut microbes of blister beetle larvae and their metabolic inferences could enrich our understanding of the nutritional requirements of the specialist and help optimize the artificial diet of medicinal cantharides.
昆虫肠道微生物群及其代谢产物在塑造宿主的饮食和摄食习惯方面发挥着重要作用。我们对以蝗虫卵和人工饲料为食的不同龄期的专食性斑蝥幼虫的肠道微生物群进行了16S rDNA扩增子测序，以探讨肠道微生物群与斑蝥幼虫专食性摄食习惯之间的关系。在相同饲养条件下，二龄至四龄幼虫的肠道微生物结构没有显著差异，但一龄幼虫的肠道微生物结构与以不同饲料喂养的二龄至四龄幼虫有显著差异。与多糖利用相关的细菌在一龄幼虫中相对较少。与人工饲料和蝗虫卵中的碳水化合物含量相比，我们推测人工饲料中过量的多糖可能对一龄幼虫的生长发育有害。以不同饲料喂养的二龄至四龄幼虫的肠道微生物群在微生物群落结构上有显著差异。不同的细菌，特别是以蝗虫卵喂养的幼虫中与代谢相关的肠道细菌，可能有助于宿主适应环境并促进活性成分的产生。与以蝗虫卵喂养的幼虫相比，以人工饲料喂养的幼虫中与多糖利用相关的细菌的相对丰度显著更高，与一龄幼虫相比也呈现相同的结果。斑蝥幼虫肠道微生物的变化及其代谢推断可以丰富我们对专食性昆虫营养需求的理解，并有助于优化药用斑蝥的人工饲料。
