Reciprocal restriction fragment length polymorphism (RFLP) analysis reveals mitochondrial heteroplasmy in hybrids.

Although mitochondria are typically inherited maternally, exceptions exist. We previously demonstrated that within-species crosses of result in paternal mitochondrial transmission, and it would be useful to know whether hybrids have only paternal mitochondria (homoplasmy) or paternal and maternal mitochondria (heteroplasmy). We developed a reciprocal restriction fragment length polymorphism analysis to separately detect paternal and maternal mitochondrial DNA. Using new hybrid lines, this approach revealed that some hybrids are heteroplasmous and others have become homoplasmous for the paternal mitotype. These results motivate additional investigation of how paternal mitochondrial transmission is apparently facile in .