Design, Synthesis, and Bioactivity Determination of Novel Malononitrile Derivatives Containing 1,2,3-Triazole.

Using antifungal agrochemicals as the most economical solution might reduce plant diseases caused by pathogenic fungi, which have a significant negative impact on the quality and yield of food worldwide. In this work, 33 compounds () containing 1,2,3-triazole and malononitrile structures were synthesized. When the compounds were tested against six fungal species, they exhibited significant fungicidal activity toward and . Compounds and displayed promising efficacy, with an EC of 0.19 and 0.27 mg/L respectively against . Fungal ergosterol production was suppressed by compounds and , according to a preliminary analysis of their mechanism of action on using transcriptomics and scanning electron microscopy. It has been shown through experimentation that compounds and prevent from synthesizing ergosterol. Ultimately, it was anticipated that compounds and would be discovered to be low-toxic.