Nishihara Y
Br J Ind Med. 1985 Feb;42(2):128-32. doi: 10.1136/oem.42.2.128.
A comparative study of the effects of biphenyl and Kanechlor-400 (KC-400) on the respiratory and energy linked activities of rat liver mitochondria was made, and some differences in effects caused by the chlorination of biphenyl were clarified. The inhibition of state 3 respiration with succinate by biphenyl was less than that observed with alpha-ketoglutarate/malate. By contrast, KC-400 exhibited the opposite trend; state 3 respiration with succinate was more sensitive to inhibition than that observed with alpha-ketoglutarate/malate. Thus the inhibition of state 3 respiration with NAD+-linked substrate was decreased, whereas the inhibition of state 3 respiration with succinate was increased by the chlorination of aromatic rings. Biphenyl also instantaneously stimulated state 4 respiration. The extent of stimulation with succinate by biphenyl was larger than with alpha-ketoglutarate-malate. On the other hand, there was about a 1-2 minute lag period before stimulation of state 4 respiration by KC-400 became obvious. Furthermore, state 4 respiration in the presence of alpha-ketoglutarate/malate was more intensely stimulated by KC-400 than by succinate. Biphenyl and KC-400 dissipated the membrane potential across the mitochondrial membranes. The dissipation of membrane potential by biphenyl was instantaneous whereas that caused by KC-400 was preceded by a lag period (1-2 min). Biphenyl and KC-400 altered the permeability properties of mitochondrial membranes as evidenced by the release of endogenous K+. The release of K+ due to biphenyl was instantaneous but KC-400 induced K+-release was preceded by a lag period (1-2 min). Thus membrane perturbation by biphenyl was faster than that induced by KC-400. Therefore, it is clear that the chlorination of aromatic rings delays the perturbation in the state of membrane lipids.
对联苯和氯丹-400（KC - 400）对大鼠肝线粒体呼吸及能量相关活性的影响进行了比较研究，明确了联苯氯化作用所导致的一些效应差异。联苯对琥珀酸介导的状态3呼吸的抑制作用小于对α-酮戊二酸/苹果酸介导的状态3呼吸的抑制作用。相比之下，KC - 400呈现出相反的趋势；琥珀酸介导的状态3呼吸比α-酮戊二酸/苹果酸介导的状态3呼吸对抑制作用更敏感。因此，芳香环氯化后，NAD⁺连接底物介导的状态3呼吸抑制作用降低，而琥珀酸介导的状态3呼吸抑制作用增强。联苯还能瞬间刺激状态4呼吸。联苯对琥珀酸介导的状态4呼吸的刺激程度大于对α-酮戊二酸 - 苹果酸介导的状态4呼吸的刺激程度。另一方面，KC - 400对状态4呼吸的刺激在大约1 - 2分钟的延迟期后才变得明显。此外，α-酮戊二酸/苹果酸存在时的状态4呼吸受KC - 400的刺激比受琥珀酸的刺激更强烈。联苯和KC - 400使线粒体膜两侧的膜电位消散。联苯导致的膜电位消散是瞬间的，而KC - 400导致的膜电位消散之前有一个延迟期（1 - 2分钟）。联苯和KC - 400改变了线粒体膜的通透性，这可由内源性K⁺的释放证明。联苯导致的K⁺释放是瞬间的，但KC - 400诱导的K⁺释放之前有一个延迟期（1 - 2分钟）。因此，联苯引起的膜扰动比KC - 400引起的更快。所以，很明显芳香环氯化会延迟膜脂质状态的扰动。
