Vovyleva-Guarriero V B, Wehbie R S, Muscatello U, Lardi G A
Biokhimiia. 1991 Mar;56(3):542-51.
Depletion of endogenous malate by preincubation of mitochondria at 30 degrees C in substrate-free media sharply decreases the rate of citrate oxidation and inhibits mitochondrial respiration in the presence of pyruvate and alpha-ketoglutarate. Addition of catalytic amounts of endogenous malate and its production via succinate oxidation promote rapid oxidation of citrate and pyruvate in the mitochondria and abolishes the lag period with alpha-ketoglutarate Malate increases the rate of membrane potential generation after addition of citrate, pyruvate or alpha-ketoglutarate to mitochondrial suspensions. Studies with controlled malate concentrations revealed that the changes in malate concentrations observed in the mitochondria in the presence of gluconeogenesis-inducing hormones may be due to the influence of these hormones on mitochondrial oxidation.
在无底物培养基中于30℃对线粒体进行预孵育，耗尽内源性苹果酸，会显著降低柠檬酸氧化速率，并在丙酮酸和α-酮戊二酸存在的情况下抑制线粒体呼吸。添加催化量的内源性苹果酸及其通过琥珀酸氧化产生，可促进线粒体中柠檬酸和丙酮酸的快速氧化，并消除α-酮戊二酸的延迟期。向线粒体悬浮液中添加柠檬酸、丙酮酸或α-酮戊二酸后，苹果酸会增加膜电位产生的速率。对苹果酸浓度进行控制的研究表明，在存在糖异生诱导激素的情况下，线粒体中观察到的苹果酸浓度变化可能是由于这些激素对线粒体氧化的影响。
