Yin Bangjie, Zeng Zihan, Zeng Hui, Hu Honghua, Zhang Min
School of Life and Environmental Sciences, Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China.
Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial Peoples Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan 610072, China.
Anal Chem. 2024 Sep 10;96(36):14348-14353. doi: 10.1021/acs.analchem.4c03189. Epub 2024 Aug 26.
On-capillary ultraviolet photometric detection (UV-PD) in a multiwavelength mode provides comprehensive analytical information. However, achieving single-point multiwavelength UV-PD for capillary-scale instruments typically requires high-cost and complex devices. This study presents the development of a cost-effective, open-source absorbance detector for on-capillary multiwavelength detection. The detector employs three deep UV light-emitting diodes emitting at 235, 255, and 278 nm as light sources, each coupled with its own photodetector for independent detection channels. The components are housed using 3D-printed parts, with an Arduino board used for data acquisition. Three individual optical paths, formed by three slits (60 μm width ×1 mm length), surround the measured capillary and converge at the same detection point. The detector demonstrates simultaneous multiwavelength detection of medicines for both HPLC and CE. This development represents an advancement in portable, low-cost analytical instrumentation, with broad implications for various fields of application.
多波长模式下的毛细管紫外光度检测（UV-PD）可提供全面的分析信息。然而，要在毛细管规模的仪器上实现单点多波长UV-PD，通常需要高成本且复杂的设备。本研究展示了一种用于毛细管多波长检测的经济高效的开源吸光度检测器的开发。该检测器采用三个分别发射235、255和278 nm的深紫外发光二极管作为光源，每个光源都与自己的光电探测器耦合，形成独立的检测通道。这些组件使用3D打印部件进行封装，并使用Arduino板进行数据采集。由三个狭缝（60μm宽×1mm长）形成的三条独立光路环绕被测毛细管，并在同一检测点汇聚。该检测器可对HPLC和CE的药物进行同步多波长检测。这一进展代表了便携式低成本分析仪器的进步，对各个应用领域具有广泛的意义。
