Caspian Faculty of Engineering, College of Engineering, University of Tehran, P.O. Box 43841-119, Guilan, Rezvanshar, Iran.
Caspian Faculty of Engineering, College of Engineering, University of Tehran, P.O. Box 43841-119, Guilan, Rezvanshar, Iran.
Int J Biol Macromol. 2024 Nov;279(Pt 2):134938. doi: 10.1016/j.ijbiomac.2024.134938. Epub 2024 Aug 24.
Effective wound management presents a substantial financial and time-related obstacle for healthcare institutions. Enhancing healthcare involves implementing innovative wound treatment methods to minimize healing time and expenses. This study is centered on the development of a non-toxic wound dressing using only two natural polymers and an enzyme. By adding 10 % wt microbial transglutaminase, the mechanical properties of the dressing were improved. This formulation increased the swelling rate by 70 %, deswelling rate by 15 %, conversion rate by 9 %, and networking rate by 20 %. Additionally, the non-toxic dressing showed a cell viability rate of 106 %. In drug delivery tests, explosive release behavior was observed, which is advantageous for open wounds. Cell staining experiments were also carried out to evaluate wound behavior in terms of collagen formation, granulation, and inflammation. The results suggest that the optimized hydrogel has great potential as a wound dressing. Its excellent absorption, antioxidant, and biocompatibility characteristics enhance tissue granulation rate and reduce wound treatment time by half compared to conventional methods, while also minimizing scarring risk. This innovative treatment, which eliminates the need for frequent changes, is beneficial for both secondary intentions and severe open wounds requiring bottom-up healing.
有效的伤口管理对医疗机构来说是一个巨大的财务和时间相关的障碍。提高医疗保健水平需要采用创新的伤口治疗方法，以最大限度地缩短愈合时间和降低费用。本研究集中于开发一种仅使用两种天然聚合物和一种酶的无毒伤口敷料。通过添加 10%wt 的微生物转谷氨酰胺酶，可以改善敷料的机械性能。该配方使敷料的溶胀率增加了 70%，脱水率降低了 15%，转化率提高了 9%，网络率提高了 20%。此外，无毒敷料的细胞存活率达到了 106%。在药物释放测试中，观察到了爆炸释放行为，这有利于开放性伤口。还进行了细胞染色实验，以评估胶原形成、肉芽形成和炎症方面的伤口行为。结果表明，优化后的水凝胶具有作为伤口敷料的巨大潜力。与传统方法相比，它具有出色的吸收性、抗氧化性和生物相容性，可将组织肉芽形成率提高一倍，并将伤口治疗时间缩短一半，同时最大限度地降低疤痕形成的风险。这种创新的治疗方法无需频繁更换敷料，有利于二期愈合和需要自下而上愈合的严重开放性伤口。
