Ny T, Bjersing L, Hsueh A J, Loskutoff D J
Endocrinology. 1985 Apr;116(4):1666-8. doi: 10.1210/endo-116-4-1666.
Although treatment of cultured granulosa cells with gonadotropins increases their fibrinolytic activity, the biochemical nature of this effect is unclear. We have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fibrin autography techniques to characterize the fibrinolytic components secreted by granulosa cells. The fibrinolytic activity of these cells results from the production of both a tissue-type plasminogen activator (t-PA) and a urokinase-like activator (u-PA). The cells also produce an inhibitor of fibrinolysis (antiactivator). FSH and LH stimulate t-PA activity and suppress antiactivator activity, while u-PA activity is not affected by the gonadotropins. The differential regulation of these molecules by the gonadotropins may be essential for ovulation.
尽管用促性腺激素处理培养的颗粒细胞会增加其纤溶活性，但这种作用的生化性质尚不清楚。我们使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和纤维蛋白自显影技术来表征颗粒细胞分泌的纤溶成分。这些细胞的纤溶活性源于组织型纤溶酶原激活剂（t-PA）和尿激酶样激活剂（u-PA）的产生。细胞还产生纤溶抑制物（抗激活剂）。促卵泡激素（FSH）和促黄体生成素（LH）刺激t-PA活性并抑制抗激活剂活性，而u-PA活性不受促性腺激素影响。促性腺激素对这些分子的差异调节可能对排卵至关重要。
