一种175千道尔顿膜蛋白的纯化、其在平滑肌和心肌中的定位以及与细胞骨架蛋白纽蛋白的相互作用。

Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles. Interaction with cytoskeletal protein - vinculin.

作者信息

Koteliansky V E, Gneushev G N, Belkin A M

出版信息

FEBS Lett. 1985 Mar 11;182(1):67-72. doi: 10.1016/0014-5793(85)81155-8.

Abstract

A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinculin immobilized on polysterene surface. It is suggested that this 175-kDa protein may be involved in physical connection between microfilaments and cell membrane.

摘要

从鸡胗平滑肌中分离出一种新的175千道尔顿膜蛋白。针对175千道尔顿蛋白的抗体被用于该蛋白在平滑肌和心肌中的定位。在这两种类型的肌肉中，175千道尔顿蛋白都定位在质膜附近。175千道尔顿蛋白能够与固定在聚苯乙烯表面的纽蛋白特异性相互作用。有人认为，这种175千道尔顿蛋白可能参与微丝与细胞膜之间的物理连接。