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[铜绿假单胞菌PAO对非溶原性噬菌体抗性突变体的分离与分析]

[Isolation and analysis of Pseudomonas aeruginosa PAO mutants resistant to nonlysogenic bacteriophages].

作者信息

Kulakov L A, Mazepa V N, Boronin A M

出版信息

Genetika. 1985 Jan;21(1):39-45.

PMID:3918919
Abstract

Nonlysogenizing Pseudomonas aeruginosa PAO bacteriophages were studied. According to morphology of the plaques, they were distributed into three groups: phi k, phi m and phi mn. The mutants of P. aeruginosa PAO resistant to these bacteriophages were selected. On the basis of cris-cross resistance analysis of the mutants, a formal scheme of the receptor sites on the P. aeruginosa PAO bacterial cell surface is drawn. It is shown that bacteriophages phi k and phi m use different receptors for their adsorption. The receptors of phi m and phi mn phages are specifically interconnected. Thus, the receptor for phi k phages is connected with the receptor for phage phi 11. It appears that the receptor for bacteriophage E79 is identical to those of phi m phages. The phi m receptor is of a composite structure: it includes two different receptors used by phi mn phages.

摘要

对非溶源性铜绿假单胞菌PAO噬菌体进行了研究。根据噬菌斑的形态,它们被分为三组:φk、φm和φmn。筛选出了对这些噬菌体具有抗性的铜绿假单胞菌PAO突变体。基于对突变体的交叉抗性分析,绘制了铜绿假单胞菌PAO细菌细胞表面受体位点的形式图。结果表明,噬菌体φk和φm利用不同的受体进行吸附。噬菌体φm和φmn的受体存在特异性联系。因此,噬菌体φk的受体与噬菌体φ11的受体相连。似乎噬菌体E79的受体与噬菌体φm的受体相同。噬菌体φm的受体具有复合结构:它包括噬菌体φmn使用的两种不同受体。

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