Tommeurd Wantanee, Thueng-In Kanyarat, Theerawatanasirikul Sirin, Tuyapala Nongnaput, Poonsuk Sukontip, Petcharat Nantawan, Thangthamniyom Nattarat, Lekcharoensuk Porntippa
Interdisciplinary Graduate Program in Genetic Engineering, The Graduate School, Kasetsart University, Bangkok 10900, Thailand.
School of Pathology, Translational Medicine Program, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Antibodies (Basel). 2024 Aug 7;13(3):67. doi: 10.3390/antib13030067.
Foot-and-mouth disease (FMD) is a highly infectious disease of cloven-hoofed animals with a significant economic impact. Early diagnosis and effective prevention and control could reduce the spread of the disease which could possibly minimize economic losses. Epitope characterization based on monoclonal antibodies provide essential information for developing diagnostic assays and vaccine designs. In this study, monoclonal antibodies raised against FMD virus (FMDV) were produced. Sixty-six monoclonal antibodies demonstrated strong reactivity and specificity to FMDV. The purified monoclonal antibodies were further used for bio-panning to select phage expressing specific epitopes from phage-displayed 12 mer-peptide library. The phage peptide sequences were analyzed using multiple sequence alignment and evaluated by peptide ELISA. Two hybridoma clones secreted monoclonal antibodies recognizing linear epitopes on VP2 of FMDV serotype O. The non-neutralizing monoclonal antibody 6F4.D11.B6 recognized the residues 67-78 on antigenic site 2 resinding in VP2, while the neutralizing monoclonal antibody 8D6.B9.C3 recognized a novel linear epitope encompassing residues 115-126 on VP2. This information and the FMDV-specific monoclonal antibodies provide valuable sources for further study and application in diagnosis, therapeutics and vaccine designs to strengthen the disease prevention and control measures.
口蹄疫（FMD）是偶蹄动物的一种高度传染性疾病，具有重大经济影响。早期诊断以及有效的预防和控制可减少疾病传播，进而可能将经济损失降至最低。基于单克隆抗体的表位特征分析可为开发诊断检测方法和疫苗设计提供重要信息。在本研究中，制备了针对口蹄疫病毒（FMDV）的单克隆抗体。66种单克隆抗体对口蹄疫病毒表现出强反应性和特异性。纯化后的单克隆抗体进一步用于生物淘选，以从噬菌体展示的12肽文库中筛选表达特定表位的噬菌体。使用多序列比对分析噬菌体肽序列，并通过肽ELISA进行评估。两个杂交瘤克隆分泌识别口蹄疫病毒O型VP2上线性表位的单克隆抗体。非中和性单克隆抗体6F4.D11.B6识别VP2上抗原位点2中第67 - 78位残基，而中和性单克隆抗体8D6.B9.C3识别包含VP2上第115 - 126位残基的新型线性表位。这些信息以及口蹄疫病毒特异性单克隆抗体为进一步研究以及在诊断、治疗和疫苗设计中的应用提供了宝贵资源，以加强疾病预防和控制措施。
