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一种用于检测鸡卵的自动化粪便卵计数系统。

An automated faecal egg count system for detection of ova in chickens.

机构信息

Parasight System, Inc., Suite 2130, Lexington, Kentucky, USA.

Wilson Veterinary Co., Needham, Indiana, USA.

出版信息

J Helminthol. 2024 Aug 27;98:e49. doi: 10.1017/S0022149X24000373.

Abstract

Chicken production has increased over the past decade, resulting in a concomitant rise in the demand for more humane options for poultry products including cage-free, free-range, and organic meat and eggs. These husbandry changes, however, have come hand-in-hand with increased prevalence of infection, which can cause clinical disease in chickens as well as the occasional appearance of worms in eggs. Additionally, development of anthelmintic resistance in closely related helminths of turkeys highlights the need for closely monitored anthelmintic treatment programs. Manual faecal egg counts (FECs) can be time-consuming and require specialist training. As such, this study sought to validate an automated FEC system for use in detection and quantification of eggs in chicken faeces. Automated counts using the Parasight System (PS) were compared to traditional manual McMaster counting for both precision and correlation between methods. Overall, ten repeated counts were performed on twenty individual samples for a total of 200 counts performed for each method. A strong, statistically significant correlation was found between methods (R = 0.7879, < 0.0001), and PS counted more eggs and performed with statistically significant higher precision ( = 0.0391) than manual McMaster counting. This study suggests that PS is a good alternative method for performing FECs and provides a new tool for use in helminth treatment and control programs in chicken operations.

摘要

过去十年间,鸡肉产量有所增加,这也导致人们对禽类产品提出了更多人性化的需求,包括无笼养、散养和有机肉类和蛋类。然而,这些饲养方式的改变伴随着 感染的流行率增加,这可能导致鸡只出现临床疾病,偶尔还会在鸡蛋中出现蠕虫。此外,火鸡中与密切相关的蠕虫出现驱虫剂耐药性,凸显出需要密切监测驱虫剂治疗方案。手动粪便卵囊计数(FEC)可能很耗时,并且需要专业培训。因此,本研究旨在验证一种用于检测和定量鸡粪便中 卵囊的自动化 FEC 系统。使用 Parasight 系统(PS)进行的自动计数与传统的手动麦克马斯特计数进行了比较,以评估其在精密度和相关性方面的表现。总体而言,对二十个个体样本进行了十次重复计数,每个方法共进行了 200 次计数。两种方法之间存在很强的统计学显著相关性(R = 0.7879,<0.0001),PS 计数的卵囊数量更多,并且具有统计学上显著更高的精密度(= 0.0391)。本研究表明,PS 是一种进行 FEC 的良好替代方法,为鸡场的蠕虫治疗和控制计划提供了一种新的工具。

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