在硒水平中等的健康人群中，解读血清谷胱甘肽过氧化物酶3（GPx-3）和硒蛋白P中硒分布与心血管危险因素的关联：阿提卡研究

Deciphering the associations of selenium distribution in serum GPx-3 and selenoprotein P with cardiovascular risk factors in a healthy population with moderate levels of selenium: The ATTICA study.

作者信息

Letsiou Sophia, Damigou Evangelia, Nomikos Tzortzis, Pergantis Spiros A, Pitsavos Christos, Panagiotakos Demosthenes, Antonopoulou Smaragdi

机构信息

Department of Nutrition and Dietetics, School of Health Sciences and Education, Harokopio University, Athens, Greece.

Department of Chemistry, University of Crete, Heraklio, Crete, Greece.

出版信息

J Trace Elem Med Biol. 2024 Dec;86:127509. doi: 10.1016/j.jtemb.2024.127509. Epub 2024 Aug 15.

DOI:10.1016/j.jtemb.2024.127509

PMID:39190954

Abstract

BACKGROUND

Selenium (Se) is an essential micronutrient, important for human health. The relationship of Se with cardiovascular risk factors is still inconclusive, especially regarding the role of different selenoproteins. The present study evaluated the relation of total serum Se as well as its distribution in plasma selenoproteins, namely glutathione peroxidase 3 (GPx3) and selenoprotein P (SelP) with cardiovascular risk factors in a sex-specific manner, in a healthy population with moderate levels of Se.

METHODS

A sub-sample from the ATTICA Study's database, consisting of 398 participants (160 females and 238 males) with data on Se and selenoproteins levels, was considered. GPx3, SelP and the main non-specific serum selenium containing protein, selenoalbumin (SeAlb) were simultaneously determined in human plasma by high-performance liquid chromatography (HPLC) coupled with inductively coupled plasma mass spectrometry (ICP-MS) at baseline.

RESULTS

Participants that belong to the highest tertiles of GPx3 and SelP presented the lowest blood pressure. Homocysteine was inversely associated with SelP and its ratio SelP/TSe in both sexes. In males, the lowest tertile of GPx3 showed lower adiponectin levels (0.66 ± 0.21 μg/mL) in comparison to the 2nd tertile of GPx3 (p=0.002), SelP was inversely associated with visceral adipose index (VAI) (-2.29 ± 0.81, p=0.005). Particularly, in males, the middle tertile of SelP had the lowest VAI values. Regarding females, lower Lp(a) concentration by 11.96 ± 5.84 mg/dL was observed in low SelP levels while higher leptin concentration by 2.30 ± 0.73 μg/L and lower fibrinogen concentration by 27.32 ± 13.30 mg/dL was detected in low GPx3 levels.

CONCLUSION

Circulating selenoproteins exert differentiated effects on cardiovascular risk factors, some of them in a sex-specific manner.

摘要

背景

硒（Se）是一种必需的微量营养素，对人体健康至关重要。硒与心血管危险因素之间的关系仍无定论，尤其是不同硒蛋白的作用。本研究以性别特异性方式评估了血清总硒及其在血浆硒蛋白（即谷胱甘肽过氧化物酶3（GPx3）和硒蛋白P（SelP））中的分布与心血管危险因素之间的关系，研究对象为硒水平适中的健康人群。

方法

考虑了来自阿提卡研究数据库的一个子样本，该样本由398名参与者（160名女性和238名男性）组成，他们有关于硒和硒蛋白水平的数据。在基线时，通过高效液相色谱（HPLC）结合电感耦合等离子体质谱（ICP-MS）同时测定人血浆中的GPx3、SelP和主要的非特异性含硒血清蛋白硒白蛋白（SeAlb）。

结果

属于GPx3和SelP最高三分位数的参与者血压最低。同型半胱氨酸与SelP及其SelP/TSe比值在两性中均呈负相关。在男性中，GPx3最低三分位数的脂联素水平（0.66±0.21μg/mL）低于GPx3第二三分位数（p=0.002），SelP与内脏脂肪指数（VAI）呈负相关（-2.29±0.81，p=0.005）。特别是在男性中，SelP的中间三分位数VAI值最低。对于女性，低SelP水平时Lp(a)浓度降低11.96±5.84mg/dL，低GPx3水平时瘦素浓度升高2.30±0.73μg/L，纤维蛋白原浓度降低27.32±13.30mg/dL。