Xiao Yu, Qin Yan, Jiang Xiaoying, Gao Pin
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China.
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China; National & Local Joint Engineering Laboratory for Municipal Sewage Resource Utilization Technology, Suzhou University of Science and Technology, Suzhou 215009, China; National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agroenvironmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou 510650, China.
Bioresour Technol. 2024 Nov;411:131358. doi: 10.1016/j.biortech.2024.131358. Epub 2024 Aug 25.
As an emerging pollutant, microplastics (MPs) have attracted increasing attention worldwide. The effects of polypropylene (PP) MPs on digestion performance, behaviors of dominant microbial communities, antibiotic resistance genes (ARGs) and mobile genetic elements in microbial anaerobic digesters were investigated. The results showed that the addition of PP-MPs to digesters led to an increase in methane production of 10.8% when 300 particles/g TSS of PP-MPs was introduced compared with that in digester not treated with PP-MPs. This increase was attributed to the enrichment of acetogens such as Syntrophobacter (42.0%), Syntrophorhabdus (27.0%), and Syntrophomonas (10.6%), and methanogens including Methanobacterium and Methanosaeta. tetX was highly enriched due to PP-MP exposure, whereas parC exhibited the greatest increase (35.5% - 222.7%). Horizontal gene transfer via ISCR1 and intI1 genes might play an important role in the spread of ARGs. Overall, these findings provide comprehensive insight into the ecological dynamics of PP-MPs during microbial anaerobic digestion.
作为一种新兴污染物，微塑料（MPs）已在全球范围内引起越来越多的关注。研究了聚丙烯（PP）微塑料对微生物厌氧消化池中消化性能、优势微生物群落行为、抗生素抗性基因（ARGs）和移动遗传元件的影响。结果表明，与未添加PP微塑料的消化池相比，当向消化池中添加300个颗粒/克总悬浮固体（TSS）的PP微塑料时，甲烷产量增加了10.8%。这种增加归因于产乙酸菌如互营杆菌属（42.0%）、互营嗜氢杆菌属（27.0%）和互营单胞菌属（10.6%）以及产甲烷菌如甲烷杆菌属和甲烷八叠球菌属的富集。由于暴露于PP微塑料，tetX高度富集，而parC的增幅最大（35.5% - 222.7%）。通过ISCR1和intI1基因的水平基因转移可能在ARGs的传播中起重要作用。总体而言，这些发现为PP微塑料在微生物厌氧消化过程中的生态动态提供了全面的见解。
