Werz W, Fischer G, Schachner M
J Neurochem. 1985 Mar;44(3):900-6. doi: 10.1111/j.1471-4159.1985.tb12901.x.
Isolated glycosaminoglycans (GAGs) were quantified biochemically in the cerebella of 6-day-old rats. 14C-Labeled hyaluronic acid (HA) and chondroitin-4-sulfate (C-4-S), added prior to isolation of GAGs from tissue, served as internal standards to allow correction for unknown losses during the purification procedure and exact quantification of GAGs in the intact tissue. Three main constituents--HA, chondroitin sulfate (CS), and heparan sulfate (HS)--were found at concentrations of 1.82, 1.52, and 0.76 micrograms/mg protein amounting to 44%, 37%, and 19% of the total GAG fraction, respectively. Incorporation of [3H]glucosamine precursor into GAGs was higher for HS (56% of incorporated precursor) and lower for HA (29%) and CS (15%). The specific activities of individual GAGs were 64.7 nCi/micrograms for HS, 14.2 for HA, and 8.3 for CS.
对6日龄大鼠的小脑进行生化分析，以定量检测分离出的糖胺聚糖（GAGs）。在从组织中分离GAGs之前添加的14C标记的透明质酸（HA）和硫酸软骨素-4（C-4-S）作为内标，用于校正纯化过程中未知的损失，并精确测定完整组织中GAGs的含量。发现三种主要成分——HA、硫酸软骨素（CS）和硫酸乙酰肝素（HS）——的浓度分别为1.82、1.52和0.76微克/毫克蛋白质，分别占总GAG组分的44%、37%和19%。[3H]葡糖胺前体掺入GAGs的比例，HS较高（占掺入前体的56%），HA较低（29%），CS更低（15%）。单个GAGs的比活性分别为：HS为64.7 nCi/微克，HA为14.2，CS为8.3。
