细胞内钙离子光学标记的指标和技术综合评述

Comprehensive review of indicators and techniques for optical mapping of intracellular calcium ions.

机构信息

Department of Pediatric Intensive Care Unit, Children's Medical Center, The First Hospital of Jilin University, Changchun, Jilin 130021, China.

State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, No. 2699, Qianjin Street, Changchun, Jilin 130012, China.

出版信息

Cereb Cortex. 2024 Aug 1;34(8). doi: 10.1093/cercor/bhae346.

DOI:10.1093/cercor/bhae346

PMID:39191664

Abstract

Calcium ions (Ca2+) play crucial roles in almost every cellular process, making the detection of changes in intracellular Ca2+ essential to understanding cell function. The fluorescence indicator method has garnered widespread application due to its exceptional sensitivity, rapid analysis, cost-effectiveness, and user-friendly nature. It has successfully delineated the spatial and temporal dynamics of Ca2+ signaling across diverse cell types. However, it is vital to understand that different indicators have varying levels of accuracy, sensitivity, and stability, making choosing the right inspection method crucial. As optical detection technologies advance, they continually broaden the horizons of scientific inquiry. This primer offers a systematic synthesis of the current fluorescence indicators and optical imaging modalities utilized for the detection of intracellular Ca2+. It elucidates their practical applications and inherent limitations, serving as an essential reference for researchers seeking to identify the most suitable detection methodologies for their calcium-centric investigations.

摘要

钙离子（Ca2+）在几乎所有细胞过程中都起着至关重要的作用，因此检测细胞内 Ca2+的变化对于理解细胞功能至关重要。荧光指示剂法由于其极高的灵敏度、快速分析、成本效益和用户友好性而得到了广泛的应用。它已经成功描绘了不同细胞类型中 Ca2+信号的时空动态。然而，必须要理解的是，不同的指示剂具有不同的准确性、灵敏度和稳定性，因此选择正确的检测方法至关重要。随着光学检测技术的进步，它们不断拓宽科学研究的视野。本指南系统地综合了目前用于检测细胞内 Ca2+的荧光指示剂和光学成像方式。它阐明了它们的实际应用和内在局限性，是那些希望为其钙相关研究确定最合适的检测方法的研究人员的重要参考。