Zhou Yong-Qing, Su Xiao-Yu, Zhao Qian, Deng Zhao-Qun
Laboratory Center, The Affiliated People's Hospital of Jiangsu University, The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City, Zhenjiang 212002, Jiangsu Province, China.
Laboratory Center, The Affiliated People's Hospital of Jiangsu University, The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City, Zhenjiang 212002, Jiangsu Province, China. E-mail：
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2024 Aug;32(4):1018-1025. doi: 10.19746/j.cnki.issn.1009-2137.2024.04.007.
To investigate the expression level, clinical significance and function of circular RNAs (circRNAs) circ_0073585 in the bone marrow of patients with acute myeloid leukemia (AML).
The expression levels of circ_0073585 in bone marrow samples of 106 newly diagnosed AML patients and 38 controls were detected by real-time quantitative PCR (RQ-PCR). The differences were compared between the two groups and their clinical significance was analyzed. The diagnostic value of circ_0073585 expression for AML was evaluated by receiver operating characteristic curve(ROC). THP-1 cells with lentivirus overexpressing circ_0073585 vector and empty vector were divided into two groups: circ_0073585-THP-1 and NC-THP-1 group. CCK-8 assay and flow cytometry were used to study the effects of circ_0073585 on THP-1 cell proliferation, survival, apoptosis and drug sensitivity.
Compared with the controls, the expression level of circ_0073585 in the bone marrow of AML patients was significantly reduced ( < 0.001). There was a statistically significant difference between the high and low expression groups of circ_0073585 in the white blood cell count, platelet count ( < 0.01) and chromosome risk ( < 0.05). Compared with NC-THP-1 cells, the proliferation and viability of circ_0073585-THP-1 cells were weakened ( < 0.01), the apoptosis rate was increased ( < 0.01), and the sensitivity to homoharringtonine ( < 0.05) and daunorubicin hydrochloride ( < 0.001) was increased.
The expression level of circ_0073585 is decreased in AML patients. Overexpression of circ_0073585 can inhibit the proliferation and viability of leukemic cells, promote apoptosis, and increase sensitivity of leukemia cells to chemotherapy drugs.
探讨环状RNA（circRNA）circ_0073585在急性髓系白血病（AML）患者骨髓中的表达水平、临床意义及功能。
采用实时定量PCR（RQ-PCR）检测106例新诊断AML患者和38例对照者骨髓样本中circ_0073585的表达水平。比较两组间差异并分析其临床意义。通过绘制受试者工作特征曲线（ROC）评估circ_0073585表达对AML的诊断价值。将过表达circ_0073585载体的慢病毒和空载体转染至THP-1细胞，分为两组：circ_0073585-THP-1组和NC-THP-1组。采用CCK-8法和流式细胞术研究circ_0073585对THP-1细胞增殖、存活、凋亡及药物敏感性的影响。
与对照组相比，AML患者骨髓中circ_0073585的表达水平显著降低（<0.001）。circ_0073585高表达组与低表达组在白细胞计数、血小板计数（<0.01）及染色体风险（<0.05）方面存在统计学差异。与NC-THP-1细胞相比，circ_0073585-THP-1细胞的增殖和活力减弱（<0.01），凋亡率升高（<0.01），对高三尖杉酯碱（<0.05）和柔红霉素（<0.001）的敏感性增加。
AML患者中circ_0073585表达水平降低。circ_0073585过表达可抑制白血病细胞的增殖和活力，促进凋亡，并增加白血病细胞对化疗药物的敏感性。
